Antibody Name:	Anti-PSMA2 Antibody
Antibody SKU:	CAB2504
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-234 of human PSMA2 (NP_002778.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Jurkat, U-251MG, HeLa, NIH/3T3, 22Rv1, Raji, Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-234 of human PSMA2 (NP_002778.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAER GYSF SLTT FSPS GKLV QIEY ALAA VAGG APSV GIKA ANGV VLAT EKKQ KSIL YDER SVHK VEPI TKHI GLVY SGMG PDYR VLVH RARK LAQQ YYLV YQEP IPTA QLVQ RVAS VMQE YTQS GGVR PFGV SLLI CGWN EGRP YLFQ SDPS GAYF AWKA TAMG KNYV NGKT FLEK RYNE DLEL EDAI HTAI LTLK ESFE GQMT EDNI EVGI CNEA GFRR LTPT EVKD YLAA IA
Gene ID:	5683
Uniprot:	P25787
Cellular Location:	Cytoplasm, Nucleus, P-body
Calculated MW:	25kDa
Observed MW:	26kDa

Synonyms:

PSMA2, HC3, MU, PMSA2, PSC2

Background:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

UniProt Protein Function:	PSMA2: a proteasomal protein of the T1A peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. The component C3 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
UniProt Protein Details:	Protein type:EC 3.4.25.1; Proteasome complex; Protease Chromosomal Location of Human Ortholog: 7p13 Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytosol; nucleus Molecular Function:threonine endopeptidase activity; protein binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; response to virus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
NCBI Summary:	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. [provided by RefSeq, Jul 2008]
UniProt Code:	P25787
NCBI GenInfo Identifier:	130850
NCBI Gene ID:	5683
NCBI Accession:	P25787.2
UniProt Related Accession:	P25787
Molecular Weight:	26kDa
NCBI Full Name:	Proteasome subunit alpha type-2
NCBI Synonym Full Names:	proteasome 20S subunit alpha 2
NCBI Official Symbol:	PSMA2
NCBI Official Synonym Symbols:	MU; HC3; PSC2; PMSA2
NCBI Protein Information:	proteasome subunit alpha type-2
UniProt Protein Name:	Proteasome subunit alpha type-2
UniProt Synonym Protein Names:	Macropain subunit C3; Multicatalytic endopeptidase complex subunit C3; Proteasome component C3
Protein Family:	Proteasome
UniProt Gene Name:	PSMA2
UniProt Entry Name:	PSA2_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMA2 antibody (CAB2504) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using PSMA2 antibody (CAB2504). Blue: DAPI for nuclear staining.