Metabolism Antibodies 2

Anti-PSAT1 Antibody (CAB6707)

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SKU:
CAB6707
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PSAT1 Antibody
Antibody SKU:CAB6707
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human PSAT1 (NP_478059.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, HeLa, SKOV3, SW620, Mouse kidney, Mouse brain, Mouse thymus, Rat kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human PSAT1 (NP_478059.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDAP RQVV NFGP GPAK LPHS VLLE IQKE LLDY KGVG ISVL EMSH RSSD FAKI INNT ENLV RELL AVPD NYKV IFLQ GGGC GQFS AVPL NLIG LKAG RCAD YVVT GAWS AKAA EEAK KFGT INIV HPKL GSYT KIPD PSTW NLNP DASY VYYC ANET VHGV EFDF IPDV KGAV LVCD MSSN FLSK PVDV SKFG VIFA GAQK NVGS AGVT VVIV RDDL LGFA LREC PSVL EYKV QAGN SSLY NTPP CFSI YVMG LVLE WIKN NGGA AAME KLSS IKSQ TIYE IIDN SQGF YVCP VEPQ NRSK MNIP FRIG NAKG DDAL EKRF LDKA LELN MLSL KGHR SVGG IRAS LYNA VTIE DVQK LAAF MKKF LEMH QL
Gene ID:29968
Uniprot:Q9Y617
Cellular Location:
Calculated MW:35kDa/40kDa
Observed MW:40kDa
Synonyms:PSAT1, EPIP, NLS2, PSA, PSAT, PSATD
Background:This gene encodes a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is a phosphoserine aminotransferase and decreased expression may be associated with schizophrenia. Mutations in this gene are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, and 8.
UniProt Protein Function:Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.
NCBI Summary:This gene encodes a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is a phosphoserine aminotransferase and decreased expression may be associated with schizophrenia. Mutations in this gene are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, and 8. [provided by RefSeq, Jul 2013]
UniProt Code:Q9Y617
NCBI GenInfo Identifier:20141815
NCBI Gene ID:29968
NCBI Accession:Q9Y617.2
UniProt Secondary Accession:Q9Y617,Q5T7G5, Q5T7G6, Q96AW2, Q9BQ12,
UniProt Related Accession:Q9Y617
Molecular Weight:41 kDa
NCBI Full Name:Phosphoserine aminotransferase
NCBI Synonym Full Names:phosphoserine aminotransferase 1
NCBI Official Symbol:PSAT1  
NCBI Official Synonym Symbols:PSA; EPIP; NLS2; PSAT; PSATD  
NCBI Protein Information:phosphoserine aminotransferase
UniProt Protein Name:Phosphoserine aminotransferase
UniProt Synonym Protein Names:Phosphohydroxythreonine aminotransferase; PSAT
Protein Family:Phosphoserine aminotransferase
UniProt Gene Name:PSAT1  
Anti-PSAT1 Antibody (CAB6707)Western blot analysis of extracts of various cell lines, using PSAT1 antibody (CAB6707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PSAT1 Antibody (CAB6707)Immunofluorescence analysis of C6 cells using PSAT1 Rabbit pAb (CAB6707) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-PSAT1 Antibody (CAB6707)Immunofluorescence analysis of HeLa cells using PSAT1 Rabbit pAb (CAB6707) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-PSAT1 Antibody (CAB6707)Immunofluorescence analysis of NIH-3T3 cells using PSAT1 Rabbit pAb (CAB6707) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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