Name: Anti-PRRX1 Antibody (CAB10237)
Brand: Cell Biology Antibodies 1
SKU: CAB10237
Availability: OutOfStock

Antibody Name:	Anti-PRRX1 Antibody
Antibody SKU:	CAB10237
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human PRRX1 (NP_073207.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse heart, Mouse brain, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human PRRX1 (NP_073207.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTSS YGHV LERQ PALG GRLD SPGN LDTL QAKK NFSV SHLL DLEE AGDM VAAQ ADEN VGEA GRSL LESP GLTS GSDT PQQD NDQL NSEE KK
Gene ID:	5396
Uniprot:	P54821
Cellular Location:	Nucleus
Calculated MW:	24kDa/27kDa
Observed MW:	27kDa

Synonyms:

PRRX1, AGOTC, PHOX1, PMX1, PRX-1, PRX1

Background:

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription co-activator, enhancing the DNA-binding activity of serum response factor, a protein required for the induction of genes by growth and differentiation factors. The protein regulates muscle creatine kinase, indicating a role in the establishment of diverse mesodermal muscle types. Alternative splicing yields two isoforms that differ in abundance and expression patterns.

UniProt Protein Function:	PRRX1: Acts as a transcriptional regulator of muscle creatine kinase (MCK) and so has a role in the establishment of diverse mesodermal muscle types. The protein binds to an A/T-rich element in the muscle creatine enhancer. Defects in PRRX1 are the cause of agnathia-otocephaly complex (AGOTC). AGOTC is a rare condition characterized by mandibular hypoplasia or agnathia, ventromedial auricular malposition (melotia) and/or auricular fusion (synotia), and microstomia with oroglossal hypoplasia or aglossia. Holoprosencephaly is the most commonly identified association, but skeletal, genitourinary, and cardiovascular anomalies, and situs inversus have been reported. The disorder is almost always lethal. Belongs to the paired homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 1q24 Cellular Component: nucleolus; nucleus Molecular Function:transcription coactivator activity Disease: Agnathia-otocephaly Complex
NCBI Summary:	The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription co-activator, enhancing the DNA-binding activity of serum response factor, a protein required for the induction of genes by growth and differentiation factors. The protein regulates muscle creatine kinase, indicating a role in the establishment of diverse mesodermal muscle types. Alternative splicing yields two isoforms that differ in abundance and expression patterns. [provided by RefSeq, Jul 2008]
UniProt Code:	P54821
NCBI GenInfo Identifier:	6174916
NCBI Gene ID:	5396
NCBI Accession:	P54821.2
UniProt Secondary Accession:	P54821,O60807, B5BUM7,
UniProt Related Accession:	P54821
Molecular Weight:	24,396 Da
NCBI Full Name:	Paired mesoderm homeobox protein 1
NCBI Synonym Full Names:	paired related homeobox 1
NCBI Official Symbol:	PRRX1
NCBI Official Synonym Symbols:	PMX1; PRX1; AGOTC; PHOX1; PRX-1
NCBI Protein Information:	paired mesoderm homeobox protein 1
UniProt Protein Name:	Paired mesoderm homeobox protein 1
UniProt Synonym Protein Names:	Homeobox protein PHOX1; Paired-related homeobox protein 1; PRX-1
Protein Family:	Paired mesoderm homeobox protein
UniProt Gene Name:	PRRX1
UniProt Entry Name:	PRRX1_HUMAN

Western blot analysis of extracts of various cell lines, using PRRX1 antibody (CAB10237) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

PRRX1 Antibody (PACO06778)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)