Antibody Name:	Anti-PRR5 Antibody
Antibody SKU:	CAB15170
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 109-388 of human PRR5 (NP_851850.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 109-388 of human PRR5 (NP_851850.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QKLL DSLA ETWD FFFS DVLP MLQA IFYP VQGK EPSV RQLA LLHF RNAI TLSV KLED ALAR AHAR VPPA IVQM LLVL QGVH ESRG VTED YLRL ETLV QKVV SPYL GTYG LHSS EGPF THSC ILEK RLLR RSRS GDVL AKNP VVRS KSYN TPLL NPVQ EHEA EGAA AGGT SIRR HSVS EMTS CPEP QGFS DPPG QGPT GTFR SSPA PHSG PCPS RLYP TTQP PEQG LDPT RSSL PRSS PENL VDQI LESV DSDS EGIF IDFG RGRG SGMS DLEG SGGR QSVV
Gene ID:	55615
Uniprot:	P85299
Cellular Location:
Calculated MW:	31kDa/32kDa/41kDa/42kDa/45kDa
Observed MW:	53kDa

Synonyms:

PRR5, FLJ20185k, PP610, PROTOR-1, PROTOR1

Background:

This gene encodes a protein with a proline-rich domain. This gene is located in a region of chromosome 22 reported to contain a tumor suppressor gene that may be involved in breast and colorectal tumorigenesis. The protein is a component of the mammalian target of rapamycin complex 2 (mTORC2), and it regulates platelet-derived growth factor (PDGF) receptor beta expression and PDGF signaling to Akt and S6K1. Alternative splicing and the use of alternative promoters results in transcripts encoding different isoforms. Read-through transcripts from this gene into the downstream Rho GTPase activating protein 8 (ARHGAP8) gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene.

UniProt Protein Function:	PROTOR1: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. May act as a tumor suppressor in breast cancer. Belongs to the PROTOR family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Tumor suppressor Chromosomal Location of Human Ortholog: 22q13 Cellular Component: TORC2 complex Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; cell cycle
NCBI Summary:	This gene encodes a protein with a proline-rich domain. This gene is located in a region of chromosome 22 reported to contain a tumor suppressor gene that may be involved in breast and colorectal tumorigenesis. The protein is a component of the mammalian target of rapamycin complex 2 (mTORC2), and it regulates platelet-derived growth factor (PDGF) receptor beta expression and PDGF signaling to Akt and S6K1. Alternative splicing and the use of alternative promoters results in transcripts encoding different isoforms. Read-through transcripts from this gene into the downstream Rho GTPase activating protein 8 (ARHGAP8) gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene. [provided by RefSeq, Nov 2010]
UniProt Code:	P85299
NCBI GenInfo Identifier:	31317218
NCBI Gene ID:	55615
NCBI Accession:	NP_851850
UniProt Secondary Accession:	P85299,O75983, O95695, Q5BIW2, Q5EAJ8, Q5EAJ9, Q5XKJ6 Q96RW1, Q96RW2, B1AHF6, B1AHG5, B3KP73,
UniProt Related Accession:	P85299
Molecular Weight:	45,172 Da
NCBI Full Name:	proline-rich protein 5 isoform 1
NCBI Synonym Full Names:	proline rich 5 (renal)
NCBI Official Symbol:	PRR5
NCBI Official Synonym Symbols:	PP610; PROTOR1; PROTOR-1; FLJ20185k
NCBI Protein Information:	proline-rich protein 5; protein observed with Rictor-1; Rho GTPase activating protein 8
UniProt Protein Name:	Proline-rich protein 5
UniProt Synonym Protein Names:	Protein observed with Rictor-1; Protor-1
Protein Family:	Proline-rich protein
UniProt Gene Name:	PRR5
UniProt Entry Name:	PRR5_HUMAN

	Western blot analysis of extracts of various cell lines, using PRR5 antibody (CAB15170) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of C6 cells using PRR5 antibody (CAB15170) at dilution of 1:100. Blue: DAPI for nuclear staining.