Epigenetics & Nuclear Signaling Antibodies 2
Anti-PRPF8 Antibody (CAB6053)
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- SKU:
- CAB6053
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-PRPF8 Antibody |
| Antibody SKU: | CAB6053 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAGV FPYR GPGN PVPG PLAP LPDY MSEE KLQE KARK WQQL QAKR YAEK RKFG FVDA QKED MPPE HVRK IIRD HGDM TNRK FRHD KRVY LGAL KYMP HAVL |
| Gene ID: | 10594 |
| Uniprot: | Q6P2Q9 |
| Cellular Location: | Nucleus speckle |
| Calculated MW: | 273kDa |
| Observed MW: | 274kDa |
| Synonyms: | PRPF8, HPRP8, PRP8, PRPC8, RP13, SNRNP220, PRPF8 |
| Background: | Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. |
| UniProt Protein Function: | Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site. |
| NCBI Summary: | Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q6P2Q9 |
| NCBI GenInfo Identifier: | 67460824 |
| NCBI Gene ID: | 10594 |
| NCBI Accession: | Q6P2Q9.2 |
| UniProt Related Accession: | Q6P2Q9 |
| Molecular Weight: | |
| NCBI Full Name: | Pre-mRNA-processing-splicing factor 8 |
| NCBI Synonym Full Names: | pre-mRNA processing factor 8 |
| NCBI Official Symbol: | PRPF8 |
| NCBI Official Synonym Symbols: | PRP8; RP13; HPRP8; PRPC8; SNRNP220 |
| NCBI Protein Information: | pre-mRNA-processing-splicing factor 8 |
| UniProt Protein Name: | Pre-mRNA-processing-splicing factor 8 |
| UniProt Synonym Protein Names: | 220 kDa U5 snRNP-specific protein; PRP8 homolog; Splicing factor Prp8; p220 |
| Protein Family: | Pre-mRNA-splicing factor |
| UniProt Gene Name: | PRPF8 |
 | Western blot analysis of extracts of various cell lines, using PRPF8 antibody (CAB6053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody (CAB6053) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody (CAB6053) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse stomach using PRPF8 antibody (CAB6053) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-251MG cells using PRPF8 antibody (CAB6053) at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining. |
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