Name: Anti-PRPF6 Antibody (CAB17122)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB17122
Availability: OutOfStock

Antibody Name:	Anti-PRPF6 Antibody
Antibody SKU:	CAB17122
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 592-941 of human PRPF6 (NP_036601.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 592-941 of human PRPF6 (NP_036601.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SLEA LLQR AVAH CPKA EVLW LMGA KSKW LAGD VPAA RSIL ALAF QANP NSEE IWLA AVKL ESEN DEYE RARR LLAK ARSS APTA RVFM KSVK LEWV QDNI RAAQ DLCE EALR HYED FPKL WMMK GQIE EQKE MMEK AREA YNQG LKKC PHST PLWL LLSR LEEK IGQL TRAR AILE KSRL KNPK NPGL WLES VRLE YRAG LKNI ANTL MAKA LQEC PNSG ILWS EAIF LEAR PQRR TKSV DALK KCEH DPHV LLAV AKLF WSQR KITK AREW FHRT VKID SDLG DAWA FFYK FELQ HGTE EQQE EVRK RCES AEPR HGEL WCAV SKDI ANWQ KKIG DILR LVAG RIKN TF
Gene ID:	24148
Uniprot:	O94906
Cellular Location:
Calculated MW:	102kDa/106kDa
Observed MW:	Refer to figures

Synonyms:	PRPF6, ANT-1, ANT1, C20orf14, Prp6, RP60, SNRNP102, TOM, U5-102K, hPrp6
Background:	The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing.

UniProt Protein Function:	PRPF6: Involved in pre-mRNA splicing. May act in the tri-snRNP complex as a bridging factor between U5 and U4/U6 snRNPs in the late step of spliceosome assembly. May be necessary for tri-snRNP formation. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation. Defects in PRPF6 may be the cause of retinitis pigmentosa type 60 (RP60). A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Cells from RP60 patients show intron retention for pre-mRNA bearing specific splicing signals. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; Nuclear receptor co-regulator; Spliceosome; RNA splicing; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 20q13.33 Cellular Component: membrane; nuclear speck; nucleoplasm; nucleus; snRNP U5; spliceosome; U4/U6 x U5 tri-snRNP complex Molecular Function:androgen receptor binding; protein binding; ribonucleoprotein binding; RNA binding; transcription coactivator activity Biological Process: assembly of spliceosomal tri-snRNP; gene expression; nuclear mRNA splicing, via spliceosome; positive regulation of transcription from RNA polymerase II promoter; RNA localization; RNA splicing; RNA splicing, via transesterification reactions; spliceosome assembly Disease: Retinitis Pigmentosa 60
NCBI Summary:	The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. [provided by RefSeq, Jul 2008]
UniProt Code:	O94906
NCBI GenInfo Identifier:	24212088
NCBI Gene ID:	24148
NCBI Accession:	O94906.1
UniProt Secondary Accession:	O94906,O95109, Q5VXS5, Q9H3Z1, Q9H4T9, Q9H4U8, Q9NTE6 B2RAR5, B3KMC6,
UniProt Related Accession:	O94906
Molecular Weight:	102,429 Da
NCBI Full Name:	Pre-mRNA-processing factor 6
NCBI Synonym Full Names:	pre-mRNA processing factor 6
NCBI Official Symbol:	PRPF6
NCBI Official Synonym Symbols:	TOM; ANT1; Prp6; RP60; ANT-1; hPrp6; U5-102K; C20orf14; SNRNP102
NCBI Protein Information:	pre-mRNA-processing factor 6
UniProt Protein Name:	Pre-mRNA-processing factor 6
UniProt Synonym Protein Names:	Androgen receptor N-terminal domain-transactivating protein 1; ANT-1; PRP6 homolog; U5 snRNP-associated 102 kDa protein; U5-102 kDa protein
Protein Family:	Pre-mRNA-processing factor
UniProt Gene Name:	PRPF6
UniProt Entry Name:	PRP6_HUMAN

Western blot analysis of extracts of HT-29 cells, using PRPF6 antibody (CAB17122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

PRPF6 Antibody (PACO03750)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)