Epigenetics & Nuclear Signaling Antibodies 2

Anti-PRPF4B Antibody (CAB14815)

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SKU:
CAB14815
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-PRPF4B Antibody
Antibody SKU:CAB14815
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 550-760 of human PRPF4B (NP_003904.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, A-549, HeLa, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 550-760 of human PRPF4B (NP_003904.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QKYK YLAE DSNM SVPS EPSS PQSS TRTR SPSP DDIL ERVA ADVK EYER ENVD TFEA SVKA KHNL MTVE QNNG SSQK KLLA PDMF TESD DMFA AYFD SARL RAAG IGKD FKEN PNLR DNWT DAEG YYRV NIGE VLDK RYNV YGYT GQGV FSNV VRAR DNAR ANQE VAVK IIRN NELM QKTG LKEL EFLK KLND ADPD DKFH CLRL FRHF YHK
Gene ID:8899
Uniprot:Q13523
Cellular Location:Nucleus
Calculated MW:116kDa
Observed MW:160kDa
Synonyms:PRPF4B, PR4H, PRP4, PRP4H, PRP4K, dJ1013A10.1
Background:Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs).
UniProt Protein Function:PRP4: a kinase of the DYRK family associated with the spliceosome and the kinetochore. It binds pre-mRNA splicing factors SFRS8, PRP6 and pinin. It is an essential kinase that associates with both the U5 snRNP and the N-CoR deacetylase complexes. It may help coordinate pre-mRNA splicing with chromatin remodeling and transcriptional regulation. PRP4 has recently been shown to be a kinetochore component that is necessary to establish a functional spindle assembly checkpoint, and is necessary for recruitment or maintenance of the checkpoint proteins RPS27, MAD1, and MAD2 at the kinetochores.
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; RNA splicing; Spliceosome; EC 2.7.11.1; Protein kinase, CMGC; CMGC group; DYRK family; PRP4 subfamily

Chromosomal Location of Human Ortholog: 6p25.2

Cellular Component: nucleoplasm

Molecular Function:protein binding; protein kinase activity

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing

NCBI Summary:Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq, Jul 2008]
UniProt Code:Q13523
NCBI GenInfo Identifier:317373526
NCBI Gene ID:8899
NCBI Accession:Q13523.3
UniProt Secondary Accession:Q13523,Q5D0F6, Q5TAY8, Q8IVC3, Q8TDP2, Q96QT7, Q9UEE6 A8K5C9,
UniProt Related Accession:Q13523
Molecular Weight:116,987 Da
NCBI Full Name:Serine/threonine-protein kinase PRP4 homolog
NCBI Synonym Full Names:pre-mRNA processing factor 4B
NCBI Official Symbol:PRPF4B  
NCBI Official Synonym Symbols:PR4H; PRP4; PRP4H; PRP4K; dJ1013A10.1  
NCBI Protein Information:serine/threonine-protein kinase PRP4 homolog
UniProt Protein Name:Serine/threonine-protein kinase PRP4 homolog
UniProt Synonym Protein Names:PRP4 kinase; PRP4 pre-mRNA-processing factor 4 homolog
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:PRPF4B  
UniProt Entry Name:PRP4B_HUMAN
Anti-PRPF4B Antibody (CAB14815)Western blot analysis of extracts of various cell lines, using PRPF4B antibody (CAB14815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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