Epigenetics & Nuclear Signaling Antibodies 2
Anti-PRPF4 Antibody (CAB6052)
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- SKU:
- CAB6052
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-PRPF4 Antibody |
| Antibody SKU: | CAB6052 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PRPF4 (NP_004688.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, SKOV3, SW480, DU145, MCF7, Mouse spleen, Mouse thymus, Mouse testis, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PRPF4 (NP_004688.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASS RASS TQAT KTKA PDDL VAPV VKKP HIYY GSLE EKER ERLA KGES GILG KDGL KAGI EAGN INIT SGEV FEIE EHIS ERQA EVLA EFER RKRA RQIN VSTD DSEV KACL RALG EPIT LFGE GPAE RRER LRNI LSVV GTDA LKKT KKDD EKSK KSKE EYQQ TWYH EGPN SLKV ARLW IANY SLPR AMKR LEEA RLHK EIPE TTRT SQMQ ELHK SLRS LNNF CSQI GDDR PISY CHFS PNSK MLAT ACWS GLCK LWSV |
| Gene ID: | 9128 |
| Uniprot: | O43172 |
| Cellular Location: | Nucleus speckle |
| Calculated MW: | 58kDa |
| Observed MW: | 58kDa |
| Synonyms: | PRPF4, HPRP4, HPRP4P, PRP4, Prp4p, RP70, SNRNP60 |
| Background: | The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | PRPF4: Involved in pre-mRNA splicing. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Spliceosome; RNA processing; RNA splicing Chromosomal Location of Human Ortholog: 9q31-q33 Cellular Component: nucleoplasm; Cajal body; spliceosome; U4/U6 x U5 tri-snRNP complex; nuclear speck; nucleus Molecular Function:U4 snRNA binding; protein binding; U6 snRNA binding Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions; RNA splicing; gene expression Disease: Retinitis Pigmentosa 70 |
| NCBI Summary: | The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | O43172 |
| NCBI GenInfo Identifier: | 20514971 |
| NCBI Gene ID: | 9128 |
| NCBI Accession: | O43172.2 |
| UniProt Secondary Accession: | O43172,O43445, O43864, Q5T1M8, Q96DG2, Q96IK4, |
| UniProt Related Accession: | O43172 |
| Molecular Weight: | 522 |
| NCBI Full Name: | U4/U6 small nuclear ribonucleoprotein Prp4 |
| NCBI Synonym Full Names: | pre-mRNA processing factor 4 |
| NCBI Official Symbol: | PRPF4 |
| NCBI Official Synonym Symbols: | PRP4; RP70; HPRP4; Prp4p; HPRP4P; SNRNP60 |
| NCBI Protein Information: | U4/U6 small nuclear ribonucleoprotein Prp4; PRP4 homolog; WD splicing factor Prp4; U4/U6 snRNP 60 kDa protein; PRP4/STK/WD splicing factor; PRP4 pre-mRNA processing factor 4 homolog |
| UniProt Protein Name: | U4/U6 small nuclear ribonucleoprotein Prp4 |
| UniProt Synonym Protein Names: | PRP4 homolog; hPrp4; U4/U6 snRNP 60 kDa protein; WD splicing factor Prp4 |
| UniProt Gene Name: | PRPF4 |
| UniProt Entry Name: | PRP4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PRPF4 antibody (CAB6052) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat lung using PRPF4 Rabbit pAb (CAB6052) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat spleen using PRPF4 Rabbit pAb (CAB6052) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human brain using PRPF4 Rabbit pAb (CAB6052) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using PRPF4 Rabbit pAb (CAB6052) at dilution of 1:200 (40x lens). |
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