Epigenetics & Nuclear Signaling Antibodies 2
Anti-PRPF31 Antibody (CAB5732)
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- SKU:
- CAB5732
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-PRPF31 Antibody |
| Antibody SKU: | CAB5732 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRPF31 (NP_056444.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, Jurkat, HepG2, Mouse lung, Mouse testis, Rat lung, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRPF31 (NP_056444.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSLA DELL ADLE EAAE EEEG GSYG EEEE EPAI EDVQ EETQ LDLS GDSV KTIA KLWD SKMF AEIM MKIE EYIS KQAK ASEV MGPV EAAP EYRV IVDA NNLT VEIE NELN IIHK FIRD KYSK RFPE LESL VPNA LDYI RTVK ELGN SLDK CKNN ENLQ QILT NATI MVVS VTAS TTQG QQLS EEEL ERLE EACD MALE LNAS |
| Gene ID: | 26121 |
| Uniprot: | Q8WWY3 |
| Cellular Location: | Cajal body, Nucleus, Nucleus speckle |
| Calculated MW: | 30kDa/40kDa/54kDa/55kDa |
| Observed MW: | 56kDa |
| Synonyms: | PRPF31, NY-BR-99, PRP31, RP11, SNRNP61 |
| Background: | This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs. |
| UniProt Protein Function: | PRPF31: Involved in pre-mRNA splicing. Required for U4/U6.U5 tri-snRNP formation. Defects in PRPF31 are the cause of retinitis pigmentosa type 11 (RP11). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP11 inheritance is autosomal dominant. Belongs to the PRP31 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA splicing; Spliceosome; RNA-binding Chromosomal Location of Human Ortholog: 19q13.42 Cellular Component: Cajal body; nuclear speck; nucleoplasm; nucleus; U4/U6 x U5 tri-snRNP complex Molecular Function:protein binding; ribonucleoprotein binding; U4 snRNA binding; U4atac snRNA binding Biological Process: assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome; snoRNA localization Disease: Retinitis Pigmentosa 11 |
| NCBI Summary: | This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.[provided by RefSeq, Jan 2009] |
| UniProt Code: | Q8WWY3 |
| NCBI GenInfo Identifier: | 90101442 |
| NCBI Gene ID: | 26121 |
| NCBI Accession: | Q8WWY3.2 |
| UniProt Secondary Accession: | Q8WWY3,Q17RB4, Q8N7F9, Q9H271, Q9Y439, E7ESA8, F1T0A4 |
| UniProt Related Accession: | Q8WWY3 |
| Molecular Weight: | 54,909 Da |
| NCBI Full Name: | U4/U6 small nuclear ribonucleoprotein Prp31 |
| NCBI Synonym Full Names: | pre-mRNA processing factor 31 |
| NCBI Official Symbol: | PRPF31 |
| NCBI Official Synonym Symbols: | RP11; PRP31; SNRNP61; NY-BR-99 |
| NCBI Protein Information: | U4/U6 small nuclear ribonucleoprotein Prp31 |
| UniProt Protein Name: | U4/U6 small nuclear ribonucleoprotein Prp31 |
| UniProt Synonym Protein Names: | Pre-mRNA-processing factor 31; Serologically defined breast cancer antigen NY-BR-99; U4/U6 snRNP 61 kDa protein; Protein 61K; hPrp31 |
| Protein Family: | U4/U6 small nuclear ribonucleoprotein |
| UniProt Gene Name: | PRPF31 |
| UniProt Entry Name: | PRP31_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PRPF31 antibody (CAB5732) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat heart using PRPF31 antibody (CAB5732) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using PRPF31 antibody (CAB5732) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using PRPF31 antibody (CAB5732) at dilution of 1:100 (40x lens). |
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