Description
Antibody Name: | Anti-PRPF31 Antibody (CAB20979) |
Antibody SKU: | CAB20979 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of Human PRPF31. |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | HeLa, HepG2, A-549, Jurkat, Mouse spleen, Rat thymus |
Immunogen: | Recombinant protein of Human PRPF31. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Cajal body, Nucleus, Nucleus speckle |
Calculated MW: | 30kDa/40kDa/54kDa/55kDa |
Observed MW: | 60KDa |
Synonyms: | PRPF31, NY-BR-99, PRP31, RP11, SNRNP61 |
Background: | This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs. |
Western blot analysis of extracts of various cell lines, using PRPF31 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s. |