Epigenetics & Nuclear Signaling Antibodies 1

Anti-PRPF18 Antibody (CAB12817)

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SKU:
CAB12817
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-PRPF18 Antibody
Antibody SKU:CAB12817
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human PRPF18 (NP_003666.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human PRPF18 (NP_003666.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDIL KSEI LRKR QLVE DRNL LVEN KKYF KRSE LAKK EEEA YFER CGYK IQPK EEDQ KPLT SSNP VLEL ELAE EKLP MTLS RQEV IRRL RERG EPIR LFGE TDYD AFQR LRKI EILT PEVN KGLR NDLK AALD KIDQ QYLN EIVG GQEP GEED TQND LKVH EENT TIEE LEAL GESL GKGD DHKD MDII TKFL KFLL GVWA KELN ARED YVKR SVQG KLNS ATQK QTES YLRP LFRK LRKR NLPA DIKE SITD IIKF MLQR EYVK ANDA YLQM AIGN APWP IGVT MVGI HART GREK IFSK HVAH VLND ETQR KYIQ GLKR LMTI CQKH FPTD PSKC VEYN AL
Gene ID:8559
Uniprot:Q99633
Cellular Location:Nucleus speckle
Calculated MW:5kDa/39kDa
Observed MW:38kDa
Synonyms:PRPF18, PRP18, hPrp18
Background:Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18.
UniProt Protein Function:PRPF18: Participates in the second step of pre-mRNA splicing. Belongs to the PRP18 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: nucleus; snRNP U5; spliceosome; U4/U6 x U5 tri-snRNP complex

Molecular Function:second spliceosomal transesterification activity

Biological Process: generation of catalytic spliceosome for second transesterification step; mRNA processing; RNA splicing

NCBI Summary:Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq, Jul 2008]
UniProt Code:Q99633
NCBI GenInfo Identifier:37082244
NCBI Gene ID:8559
NCBI Accession:Q99633.1
UniProt Secondary Accession:Q99633,Q5T9P9, Q9BUI9,
UniProt Related Accession:Q99633
Molecular Weight:40kDa
NCBI Full Name:Pre-mRNA-splicing factor 18
NCBI Synonym Full Names:pre-mRNA processing factor 18
NCBI Official Symbol:PRPF18  
NCBI Official Synonym Symbols:PRP18; hPrp18  
NCBI Protein Information:pre-mRNA-splicing factor 18
UniProt Protein Name:Pre-mRNA-splicing factor 18
UniProt Synonym Protein Names:PRP18 homolog; hPRP18
UniProt Gene Name:PRPF18  
Anti-PRPF18 Antibody (CAB12817)Western blot analysis of extracts of HeLa cells, using PRPF18 antibody (CAB12817) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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