Metabolism Antibodies 3
Anti-Protein Wnt-5b Antibody (CAB8313)
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- SKU:
- CAB8313
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | WNT5B Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8313 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-359 of human WNT5B (NP_116031.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, 293T, SH-SY5Y, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-359 of human WNT5B (NP_116031.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VSAA GVVN AISR ACRE GELS TCGC SRTA RPKD LPRD WLWG GCGD NVEY GYRF AKEF VDAR EREK NFAK GSEE QGRV LMNL QNNE AGRR AVYK MADV ACKC HGVS GSCS LKTC WLQL AEFR KVGD RLKE KYDS AAAM RVTR KGRL ELVN SRFT QPTP EDLV YVDP SPDY CLRN ESTG SLGT QGRL CNKT SEGM DGCE LMCC GRGY NQFK SVQV ERCH CKFH WCCF VRCK KCTE IVDQ YICK |
| Gene ID: | 81029 |
| Uniprot: | Q9H1J7 |
| Cellular Location: | Secreted, extracellular matrix, extracellular space |
| Calculated MW: | 40kDa |
| Observed MW: | 50kDa |
| Synonyms: | WNT5B |
| Background: | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. |
| UniProt Protein Function: | WNT5B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Interacts with PORCN. Belongs to the Wnt family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 12p13.3 Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane Molecular Function:frizzled binding; receptor binding Biological Process: cell fate commitment; chondrocyte differentiation; neuron differentiation; positive regulation of cell migration; Wnt receptor signaling pathway |
| NCBI Summary: | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H1J7 |
| NCBI GenInfo Identifier: | 20532427 |
| NCBI Gene ID: | 81029 |
| NCBI Accession: | Q9H1J7.2 |
| UniProt Secondary Accession: | Q9H1J7,Q96S49, Q9BV04, A8K315, D3DUP9, |
| UniProt Related Accession: | Q9H1J7 |
| Molecular Weight: | 40,323 Da |
| NCBI Full Name: | Protein Wnt-5b |
| NCBI Synonym Full Names: | Wnt family member 5B |
| NCBI Official Symbol: | WNT5B |
| NCBI Protein Information: | protein Wnt-5b |
| UniProt Protein Name: | Protein Wnt-5b |
| UniProt Gene Name: | WNT5B |
| UniProt Entry Name: | WNT5B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using WNT5B antibody (CAB8313) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunohistochemistry of paraffin-embedded rat lung using WNT5B antibody (CAB8313) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat bone marrow using WNT5B antibody (CAB8313) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using WNT5B antibody (CAB8313) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using WNT5B antibody (CAB8313) at dilution of 1:100 (40x lens). |
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