Immunology Antibodies 3

Anti-Protein O-GlcNAcase Antibody (CAB9033)

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SKU:
CAB9033
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-Protein O-GlcNAcase Antibody
Antibody SKU:CAB9033
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 380-580 of human MGEA5 (NP_001135906.1).
Application:WB IHC
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, A375, BxPC-3, HeLa, Mouse brain, Mouse lung, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 380-580 of human MGEA5 (NP_001135906.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YQEP IMSQ GAAL SGEP TTLT KEEE KKQP DEEP MDMV VEKQ EETD HKND NQIL SEIV EAKM AEEL KPMD TDKE SIAE SKSP EMSM QEDC ISDI APMQ TDEQ TNKE QFVP GPNE KPLY TAEP VTLE DLQL LADL FYLP YEHG PKGA QMLR EFQW LRAN SSVV SVNC KGKD SEKI EEWR SRAA KFEE MCGL VMGM FTRL SNCA N
Gene ID:10724
Uniprot:O60502
Cellular Location:Cytoplasm, Nucleus
Calculated MW:76kDa/95kDa/96kDa/102kDa
Observed MW:120kDa
Synonyms:MGEA5, MEA5, NCOAT, OGA
Background:
UniProt Protein Function:OGA: Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Acetyltransferase; Hydrolase; EC 3.2.1.169; EC 3.2.1.52

Chromosomal Location of Human Ortholog: 10q24.1-q24.3

Cellular Component: membrane; cytosol; nucleus

Molecular Function:beta-N-acetylglucosaminidase activity; histone acetyltransferase activity; hyalurononglucosaminidase activity

Biological Process: positive regulation of DNA metabolic process; positive regulation of protein complex disassembly; dATP metabolic process; positive regulation of proteolysis; protein deglycosylation; N-acetylglucosamine metabolic process; positive regulation of insulin secretion; positive regulation of growth hormone secretion; positive regulation of glucose import; negative regulation of protein amino acid glycosylation; positive regulation of mitochondrial depolarization; response to steroid hormone stimulus; positive regulation of cell killing; glycoprotein catabolic process; histone acetylation; protein targeting to membrane; aging

NCBI Summary:The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]
UniProt Code:O60502
NCBI GenInfo Identifier:215490056
NCBI Gene ID:10724
NCBI Accession:NP_001135906.1
UniProt Secondary Accession:O60502,O75166, Q86WV0, Q8IV98, Q9BVA5, Q9HAR0, B7WPB9 D3DR79, E9PGF9,
UniProt Related Accession:O60502
Molecular Weight:96,932 Da
NCBI Full Name:bifunctional protein NCOAT isoform b
NCBI Synonym Full Names:meningioma expressed antigen 5 (hyaluronidase)
NCBI Official Symbol:MGEA5  
NCBI Official Synonym Symbols:OGA; MEA5; NCOAT  
NCBI Protein Information:bifunctional protein NCOAT; O-GlcNAcase; hyaluronidase in meningioma; meningioma-expressed antigen 5; nuclear cytoplasmic O-GlcNAcase and acetyltransferase
UniProt Protein Name:Bifunctional protein NCOAT
UniProt Synonym Protein Names:Meningioma-expressed antigen 5; Nuclear cytoplasmic O-GlcNAcase and acetyltransferaseIncluding the following 2 domains:Protein O-GlcNAcase (EC:3.2.1.169)Alternative name(s):Glycoside hydrolase O-GlcNAcase; Hexosaminidase C; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; O-GlcNAcase; OGA
Protein Family:Protein O-GlcNAcase
UniProt Gene Name:MGEA5  
UniProt Entry Name:NCOAT_HUMAN
Anti-Protein O-GlcNAcase Polyclonal Antibody (CAB9033)Western blot analysis of extracts of various cell lines, using MGEA5 antibody (CAB9033) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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