Metabolism Antibodies 2

Anti-PRODH Antibody (CAB5836)

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SKU:
CAB5836
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-PRODH Antibody
Antibody SKU:CAB5836
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 321-600 of human PRODH (NP_057419.4).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse liver, Mouse kidney, Mouse heart, Rat liver, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 321-600 of human PRODH (NP_057419.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IDSR TKLS KHLV VPNA QTGQ LEPL LSRF TEEE ELQM TRML QRMD VLAK KATE MGVR LMVD AEQT YFQP AISR LTLE MQRK FNVE KPLI FNTY QCYL KDAY DNVT LDVE LARR EGWC FGAK LVRG AYLA QERA RAAE IGYE DPIN PTYE ATNA MYHR CLDY VLEE LKHN AKAK VMVA SHNE DTVR FALR RMEE LGLH PADH QVYF GQLL GMCD QISF PLGQ AGYP VYKY VPYG PVME VLPY LSRR ALEN SSLM KGTH RERQ LLWL ELLR RLRT GNLF HRPA
Gene ID:5625
Uniprot:O43272
Cellular Location:Mitochondrion matrix
Calculated MW:56kDa/59kDa/68kDa
Observed MW:68kDa
Synonyms:PRODH, HSPOX2, PIG6, POX, PRODH1, PRODH2, TP53I6
Background:This gene encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in this gene are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). This gene is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:PRODH: Converts proline to delta-1-pyrroline-5-carboxylate. Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1). HP-1 is a disorder characterized by elevated serum proline levels. May be involved in the psychiatric and behavioral phenotypes associated with the 22q11 velocardiofacial and DiGeorge syndrome. Defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4). A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. Belongs to the proline oxidase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 1.5.5.2; Mitochondrial; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: mitochondrial matrix; mitochondrial inner membrane

Molecular Function:proline dehydrogenase activity

Biological Process: proline catabolic process; 4-hydroxyproline catabolic process; induction of apoptosis by oxidative stress; proline catabolic process to glutamate; proline metabolic process

Disease: Hyperprolinemia, Type I; Schizophrenia 4

NCBI Summary:This gene encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in this gene are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). This gene is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010]
UniProt Code:O43272
NCBI GenInfo Identifier:327478561
NCBI Gene ID:5625
NCBI Accession:O43272.3
UniProt Secondary Accession:O43272,O14680, Q0P507, Q147W8, Q504W1, Q59FI8, Q6NV86 Q9UF13, A6NF53,
UniProt Related Accession:O43272
Molecular Weight:600
NCBI Full Name:Proline dehydrogenase 1, mitochondrial
NCBI Synonym Full Names:proline dehydrogenase (oxidase) 1
NCBI Official Symbol:PRODH  
NCBI Official Synonym Symbols:POX; PIG6; HSPOX2; PRODH1; PRODH2; TP53I6  
NCBI Protein Information:proline dehydrogenase 1, mitochondrial; proline oxidase 2; p53-induced gene 6 protein; proline oxidase, mitochondrial; tumor protein p53 inducible protein 6
UniProt Protein Name:Proline dehydrogenase 1, mitochondrial
UniProt Synonym Protein Names:Proline oxidase; Proline oxidase 2; p53-induced gene 6 protein
UniProt Gene Name:PRODH  
UniProt Entry Name:PROD_HUMAN
Anti-PRODH Antibody (CAB5836)Western blot analysis of extracts of various cell lines, using PRODH antibody (CAB5836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-PRODH Antibody (CAB5836)Immunohistochemistry of paraffin-embedded human liver damage using PRODH antibody (CAB5836) at dilution of 1:100 (40x lens).
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