Description
Antibody Name: | Anti-PRMT7 Antibody (CAB20975) |
Antibody SKU: | CAB20975 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of Human PRMT7. |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | HeLa, SW620, MCF7, NIH/3T3, Mouse testis, Mouse brain, Rat testis, Rat brain |
Immunogen: | Recombinant protein of Human PRMT7. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Cytoplasm, Nucleus, cytosol |
Calculated MW: | 62kDa/63kDa/73kDa/78kDa |
Observed MW: | 78KDa |
Synonyms: | PRMT7, SBIDDS |
Background: | Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 PubMed 15044439). |
Western blot analysis of extracts of various cell lines, using PRMT7 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |