Name: Anti-PRMT7 Antibody (CAB12159)
Brand: Developmental Biology
SKU: CAB12159
Availability: OutOfStock

Antibody Name:	Anti-PRMT7 Antibody
Antibody SKU:	CAB12159
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 513-692 of human PRMT7 (NP_061896.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HeLa, MCF7, Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 513-692 of human PRMT7 (NP_061896.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QAAS LHAV VVEF RDLW RIRS PCGD CEGF DVHI MDDM IKRA LDFR ESRE AEPH PLWE YPCR SLSE PWQI LTFD FQQP VPLQ PLCA EGTV ELRR PGQS HAAV LWME YHLT PECT LSTG LLEP ADPE GGCC WNPH CKQA VYFF SPAP DPRA LLGG PRTV SYAV EFHP DTGD IIME FRHA DTPD
Gene ID:	54496
Uniprot:	Q9NVM4
Cellular Location:	Cytoplasm, Nucleus, cytosol
Calculated MW:	62kDa/63kDa/73kDa/78kDa
Observed MW:	75kDa

Synonyms:

PRMT7, SBIDDS

Background:

This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients.

UniProt Protein Function:	PRMT7: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo. Belongs to the protein arginine N-methyltransferase family. PRMT7 subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.1.1.125; EC 2.1.1.126; Methyltransferase; Methyltransferase, protein arginine; RNA processing Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: cytosol; fibrillar center; nucleoplasm; nucleus Molecular Function:[myelin basic protein]-arginine N-methyltransferase activity; histone binding; histone-arginine N-methyltransferase activity; protein-arginine N-methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; protein-arginine omega-N symmetric methyltransferase activity; ribonucleoprotein binding; S-adenosylmethionine-dependent methyltransferase activity Biological Process: DNA methylation during gametogenesis; genetic imprinting; histone methylation; peptidyl-arginine methylation; regulation of protein binding; spliceosomal snRNP biogenesis Disease: Short Stature, Brachydactyly, Intellectual Developmental Disability, And Seizures
NCBI Summary:	This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients. [provided by RefSeq, May 2017]
UniProt Code:	Q9NVM4
NCBI GenInfo Identifier:	20137529
NCBI Gene ID:	54496
NCBI Accession:	Q9NVM4.1
UniProt Secondary Accession:	Q9NVM4,Q96PV5, Q9H9L0, B3KPR0, B3KUG9, B4E379,
UniProt Related Accession:	Q9NVM4
Molecular Weight:	78kDa
NCBI Full Name:	Protein arginine N-methyltransferase 7
NCBI Synonym Full Names:	protein arginine methyltransferase 7
NCBI Official Symbol:	PRMT7
NCBI Official Synonym Symbols:	SBIDDS
NCBI Protein Information:	protein arginine N-methyltransferase 7
UniProt Protein Name:	Protein arginine N-methyltransferase 7
UniProt Synonym Protein Names:	Histone-arginine N-methyltransferase PRMT7; [Myelin basic protein]-arginine N-methyltransferase PRMT7
UniProt Gene Name:	PRMT7

Western blot analysis of extracts of various cell lines, using PRMT7 antibody (CAB12159) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-PRMT7 Antibody (CAB17164)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)