Name: Anti-PRMT2 Antibody (CAB5835)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB5835
Availability: OutOfStock

Antibody Name:	Anti-PRMT2 Antibody [KO Validated]
Antibody SKU:	CAB5835
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PRMT2 (NP_996845.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PRMT2 (NP_996845.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATS GDCP RSES QGEE PAEC SEAG LLQE GVQP EEFV AIAD YAAT DETQ LSFL RGEK ILIL RQTT ADWW WGER AGCC GYIP ANHV GKHV DEYD PEDT WQDE EYFG SYGT LKLH LEML ADQP RTTK YHSV ILQN KESL TDKV ILDV GCGT GIIS LFCA HYAR PRAV YAVE ASEM AQHT GQLV LQNG FADI ITVY QQKV EDVV LPEK VDVL VSEW MGTC LLFE FMIE SILY ARDA WLKE DGVI WPTM AALH LVPC SADK DYRS KVLF WDNA YEFN LSAL KSLA VKEF FSKP KYNH ILKP EDCL
Gene ID:	3275
Uniprot:	P55345
Cellular Location:	Cytoplasm, Nucleus, Nucleus, nucleolus
Calculated MW:	25kDa/31kDa/32kDa/33kDa/37kDa/49kDa
Observed MW:	56kDa

Synonyms:	PRMT2, HRMT1L1
Background:

UniProt Protein Function:	PRMT2: Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1- dependent manner). May be involved in growth regulation. Belongs to the protein arginine N-methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Methyltransferase; Methyltransferase, protein arginine; Nuclear receptor co-regulator; EC 2.1.1.125; Nucleolus Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: nucleoplasm; Rb-E2F complex; cytoplasm; nucleolus; nucleus; cytosol Molecular Function:histone methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine N-methyltransferase activity; peroxisome proliferator activated receptor binding; protein homodimerization activity; retinoic acid receptor binding; transcription coactivator activity; histone-arginine N-methyltransferase activity; protein binding; signal transducer activity; androgen receptor binding; estrogen receptor binding; thyroid hormone receptor binding; progesterone receptor binding Biological Process: histone methylation; developmental cell growth; protein amino acid methylation; inhibition of NF-kappaB transcription factor; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; signal transduction; negative regulation of transcription, DNA-dependent
UniProt Code:	P55345
NCBI GenInfo Identifier:	2499805
NCBI Gene ID:	3275
NCBI Accession:	P55345.1
UniProt Secondary Accession:	P55345,P78350, Q498Y5, Q5U7D4, Q6FHF0, Q99781, Q9BW15 Q9UMC2, B7U630, B7U631, B7U632,
UniProt Related Accession:	P55345
Molecular Weight:	433
NCBI Full Name:	Protein arginine N-methyltransferase 2
NCBI Synonym Full Names:	protein arginine methyltransferase 2
NCBI Official Symbol:	PRMT2
NCBI Official Synonym Symbols:	HRMT1L1
NCBI Protein Information:	protein arginine N-methyltransferase 2; PRMT2 beta; PRMT2 alpha; PRMT2 gamma; HMT1 hnRNP methyltransferase-like 1; histone-arginine N-methyltransferase PRMT2; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 1
UniProt Protein Name:	Protein arginine N-methyltransferase 2
UniProt Synonym Protein Names:	Histone-arginine N-methyltransferase PRMT2 (EC:2.1.1.125)
UniProt Gene Name:	PRMT2
UniProt Entry Name:	ANM2_HUMAN

	Western blot analysis of extracts of mouse lung, using PRMT2 antibody (CAB5835) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and PRMT2 Rabbit pAb knockout (KO) 293T cells, using PRMT2 Rabbit pAb antibody (CAB5835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver damage using PRMT2 antibody (CAB5835) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using PRMT2 antibody (CAB5835).