Antibody Name:	Anti-PRMT1 Antibody
Antibody SKU:	CAB1055
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-353 of human PRMT1 (NP_938074.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, A549, rat spleen, rat lung, rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-353 of human PRMT1 (NP_938074.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SSEK PNAE DMTS KDYY FDSY AHFG IHEE MLKD EVRT LTYR NSMF HNRH LFKD KVVL DVGS GTGI LCMF AAKA GARK VIGI ECSS ISDY AVKI VKAN KLDH VVTI IKGK VEEV ELPV EKVD IIIS EWMG YCLF YESM LNTV LYAR DKWL APDG LIFP DRAT LYVT AIED RQYK DYKI HWWE NVYG FDMS CIKD VAIK EPLV DVVD PKQL VTNA CLIK EVDI YTVK VEDL TFTS PFCL QVKR NDYV HALV AYFN IEFT RCHK RTGF STSP ESPY THWK QTVF YMED YLTV KTGE EIFG TIGM RPNA KNNR DLDF TIDL DFKG QLCE LSCS TDYR MR
Gene ID:	3276
Uniprot:	Q99873
Cellular Location:	Cytoplasm, Nucleus, cytosol, nucleoplasm
Calculated MW:	39kDa/40kDa/41kDa
Observed MW:	42kDa

Synonyms:

PRMT1, ANM1, HCP1, HRMT1L2, IR1B4

Background:

This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5.

UniProt Protein Function:	PRMT1: Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Homodimer and heterodimer with PRMT8. The dimer can then associate to form a homohexamer. Interacts with ILF3, BTG1, BTG2, SUPT5H and interferon-alpha/beta receptor 1. Interacts with NFATC2IP. Widely expressed. By BTG1, BTG2 and ILF3. Belongs to the protein arginine N-methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Methyltransferase; EC 2.1.1.125; Nuclear receptor co-regulator; Methyltransferase, protein arginine Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol Molecular Function:identical protein binding; protein-arginine omega-N asymmetric methyltransferase activity; histone methyltransferase activity; methyltransferase activity; protein binding; N-methyltransferase activity Biological Process: histone methylation; peptidyl-arginine methylation; establishment and/or maintenance of chromatin architecture; cell surface receptor linked signal transduction; protein amino acid methylation; regulation of transcription, DNA-dependent; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of megakaryocyte differentiation; neurite development
NCBI Summary:	This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]
UniProt Code:	Q99873
NCBI GenInfo Identifier:	161789011
NCBI Gene ID:	3276
NCBI Accession:	Q99873.2
UniProt Secondary Accession:	Q99873,Q15529, Q2VP93, Q6LEU5, Q8WUW5, Q99872, Q99874 Q9NZ04, Q9NZ05, Q9NZ06, B4E3C3, G5E9B6,
UniProt Related Accession:	Q99873
Molecular Weight:	361
NCBI Full Name:	Protein arginine N-methyltransferase 1
NCBI Synonym Full Names:	protein arginine methyltransferase 1
NCBI Official Symbol:	PRMT1
NCBI Official Synonym Symbols:	ANM1; HCP1; IR1B4; HRMT1L2
NCBI Protein Information:	protein arginine N-methyltransferase 1; interferon receptor 1-bound protein 4; histone-arginine N-methyltransferase PRMT1; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2; heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2
UniProt Protein Name:	Protein arginine N-methyltransferase 1
UniProt Synonym Protein Names:	Histone-arginine N-methyltransferase PRMT1 (EC:2.1.1.125); Interferon receptor 1-bound protein 4
Protein Family:	Protein arginine N-methyltransferase
UniProt Gene Name:	PRMT1
UniProt Entry Name:	ANM1_HUMAN

	Western blot analysis of extracts of various cell lines, using PRMT1 antibody (CAB1055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using PRMT1 antibody (CAB1055) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using PRMT1 antibody (CAB1055) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PRMT1 antibody (CAB1055) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using PRMT1 Rabbit pAb (CAB1055) at dilution of 1:100. Blue: DAPI for nuclear staining.