Name: Anti-PRKRA Antibody (CAB5417)
Brand: Immunology Antibodies 2
SKU: CAB5417
Availability: OutOfStock

Antibody Name:	Anti-PRKRA Antibody
Antibody SKU:	CAB5417
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human PRKRA (NP_003681.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse
Positive Samples:	Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human PRKRA (NP_003681.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSQS RHRA EAPP LERE DSGT FSLG KMIT AKPG KTPI QVLH EYGM KTKN IPVY ECER SDVQ IHVP TFTF RVTV GDIT CTGE GTSK KLAK HRAA EAAI NILK ANAS ICFA VPDP LMPD PSKQ PKNQ LNPI GSLQ ELAI HHGW RLPE YTLS QEGG PAHK REYT TICR LESF METG KGAS KKQA KRNA AEKF LAKF SNIS PENH ISLT NVVG HSLG CTWH SLRN SPGE KINL LKRS LLSI PNTD YIQL LSEI AKEQ GFNI TYLD IDEL SANG QYQC LAEL STSP ITVC HGSG ISCG NAQS DAAH NALQ YLKI IAER K
Gene ID:	8575
Uniprot:	O75569
Cellular Location:	Cytoplasm, perinuclear region
Calculated MW:	31kDa/33kDa/34kDa
Observed MW:	35kDa

Synonyms:	PRKRA, DYT16, HSD14, PACT, RAX
Background:	This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	PACT: a double strand RNA binding protein (dsRBP). Interacts with TRBP and Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. Activates the interferon-induced protein kinase PKR. Three alternatively spliced human isoforms have been reported.
UniProt Protein Details:	Protein type:Activator; RNA-binding; RNA processing Chromosomal Location of Human Ortholog: 2q31.2 Cellular Component: nucleoplasm; membrane; perinuclear region of cytoplasm; cytoplasm; cytosol Molecular Function:protein binding; protein homodimerization activity; enzyme activator activity Biological Process: positive regulation of catalytic activity; negative regulation of cell proliferation; skeletal morphogenesis; response to virus; outer ear morphogenesis; immune response; gene expression; middle ear morphogenesis; RNA interference, production of siRNA; protein amino acid phosphorylation Disease: Dystonia 16
NCBI Summary:	This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]
UniProt Code:	O75569
NCBI GenInfo Identifier:	74735517
NCBI Gene ID:	8575
NCBI Accession:	O75569.1
UniProt Secondary Accession:	O75569,Q53G24, Q6X7T5, Q8NDK4, A8K3I6,
UniProt Related Accession:	O75569
Molecular Weight:	313
NCBI Full Name:	Interferon-inducible double-stranded RNA-dependent protein kinase activator A
NCBI Synonym Full Names:	protein kinase, interferon-inducible double stranded RNA dependent activator
NCBI Official Symbol:	PRKRA
NCBI Official Synonym Symbols:	RAX; PACT; DYT16; HSD14
NCBI Protein Information:	interferon-inducible double-stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PKR-associating protein X; protein activator of the interferon-induced protein kinase; protein kinase, interferon-inducible double stranded RNA-dependent activator; protein kinase, interferon-inducible double-stranded RNA-dependent activator; interferon-inducible double stranded RNA-dependent protein kinase activator A
UniProt Protein Name:	Interferon-inducible double-stranded RNA-dependent protein kinase activator A
UniProt Synonym Protein Names:	PKR-associated protein X; PKR-associating protein X; Protein activator of the interferon-induced protein kinase; Protein kinase, interferon-inducible double-stranded RNA-dependent activator
Protein Family:	Interferon-inducible double-stranded RNA-dependent protein kinase activator
UniProt Gene Name:	PRKRA
UniProt Entry Name:	PRKRA_HUMAN

Western blot analysis of extracts of mouse liver, using PRKRA antibody (CAB5417) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

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Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)