Antibody Name:	Anti-PRKG1 Antibody
Antibody SKU:	CAB2565
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PRKG1 (NP_006249.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PRKG1 (NP_006249.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGTL RDLQ YALQ EKIE ELRQ RDAL IDEL ELEL DQKD ELIQ KLQN ELDK YRSV IRPA TQQA QKQS ASTL QGEP RTKR QAIS AEPT AFDI QDLS HVTL PFYP KSPQ SKDL IKEA ILDN DFMK NLEL SQIQ EIVD CMYP VEYG KDSC IIKE GDVG SLVY VMED GKVE VTKE GVKL CTMG PGKV FGEL AILY NCTR TATV KTLV NVKL WAID RQCF QTIM MRTG LIKH TEYM EFLK SVPT FQSL PEEI LSKL ADVL EETH YENG EYII RQGA RGDT FFII SKGT VNVT REDS PSED PVFL RTLG
Gene ID:	5592
Uniprot:	Q13976
Cellular Location:	Cytoplasm
Calculated MW:	44kDa/76kDa/77kDa
Observed MW:	65kDa

Synonyms:

PRKG1, AAT8, PKG, PRKG1B, PRKGR1B, cGK, cGK 1, cGK1, cGKI, cGKI-BETA, cGKI-alpha

Background:

Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.

UniProt Protein Function:	PKG1: an AGC kinase of the PKG family. Type I cGMP-dependent protein kinase; relaxes vascular smooth muscle and inhibits platelet aggregation. Binding of cGMP results in enzyme activation. Two splice variant isoforms have been described.
UniProt Protein Details:	Protein type:AGC group; EC 2.7.11.12; Kinase, protein; PKG family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor) Chromosomal Location of Human Ortholog: 10q11.23-q21.1 Cellular Component: cytoplasm; cytosol; plasma membrane Molecular Function:calcium channel regulator activity; cGMP-dependent protein kinase activity; protein binding; protein serine/threonine kinase activity Biological Process: actin cytoskeleton organization and biogenesis; regulation of GTPase activity; signal transduction
NCBI Summary:	Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]
UniProt Code:	Q13976
NCBI GenInfo Identifier:	6225588
NCBI Gene ID:	5592
NCBI Accession:	Q13976.3
UniProt Secondary Accession:	Q13976,P14619, Q5JP05, Q5JSJ6, Q6P5T7, A5YM56, B3KSF3 E2PU10,
UniProt Related Accession:	Q13976
Molecular Weight:
NCBI Full Name:	cGMP-dependent protein kinase 1
NCBI Synonym Full Names:	protein kinase, cGMP-dependent, type I
NCBI Official Symbol:	PRKG1
NCBI Official Synonym Symbols:	PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha
NCBI Protein Information:	cGMP-dependent protein kinase 1
UniProt Protein Name:	cGMP-dependent protein kinase 1
UniProt Synonym Protein Names:	cGMP-dependent protein kinase I; cGKI
UniProt Gene Name:	PRKG1

	Western blot analysis of extracts of HepG2 cells, using PRKG1 antibody (CAB2565) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
	Immunohistochemistry of paraffin-embedded mouse lung using PRKG1 antibody (CAB2565) at dilution of 1:100 (40x lens).