Immunology Antibodies 2

Anti-PRKCZ Antibody (CAB5714)

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SKU:
CAB5714
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-PRKCZ Antibody
Antibody SKU:CAB5714
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 453-592 of human PRKCZ (NP_002735.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HT-29, Mouse lung, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 453-592 of human PRKCZ (NP_002735.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IITD NPDM NTED YLFQ VILE KPIR IPRF LSVK ASHV LKGF LNKD PKER LGCR PQTG FSDI KSHA FFRS IDWD LLEK KQAL PPFQ PQIT DDYG LDNF DTQF TSEP VQLT PDDE DAIK RIDQ SEFE GFEY INPL LLST EESV
Gene ID:5590
Uniprot:Q05513
Cellular Location:Cell junction, Cytoplasm, Endosome
Calculated MW:46kDa/56kDa/67kDa
Observed MW:70kDa
Synonyms:PRKCZ, PKC-ZETA, PKC2
Background:Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:PKCZ: an AGC kinase of the PKC family. An atypical PKC: its activity is not regulated by Ca2+, PS, DAG or phorbol esters. Constitutively active.
UniProt Protein Details:

Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.13; AGC group; PKC family; Iota subfamily

Chromosomal Location of Human Ortholog: 1p36.33-p36.2

Cellular Component: cell junction; cytoplasm; cytosol; intercellular junction; membrane; plasma membrane; vesicle

Molecular Function:insulin receptor substrate binding; protein binding; protein kinase activity; protein kinase C activity; protein serine/threonine kinase activity

Biological Process: activation of NF-kappaB transcription factor; establishment of cell polarity; negative regulation of insulin receptor signaling pathway; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of protein complex assembly; peptidyl-serine phosphorylation; positive regulation of insulin receptor signaling pathway; positive regulation of interleukin-4 production; positive regulation of T-helper 2 cell differentiation; protein amino acid phosphorylation; signal transduction; transforming growth factor beta receptor signaling pathway

NCBI Summary:Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:Q05513
NCBI GenInfo Identifier:68067736
NCBI Gene ID:5590
NCBI Accession:Q05513.4
UniProt Secondary Accession:Q05513,Q15207, Q5SYT5, Q969S4, A8K4N0, A8MU64, B7Z2J7 E9PCW2,
UniProt Related Accession:Q05513
Molecular Weight:56,136 Da
NCBI Full Name:Protein kinase C zeta type
NCBI Synonym Full Names:protein kinase C zeta
NCBI Official Symbol:PRKCZ  
NCBI Official Synonym Symbols:PKC2; PKC-ZETA  
NCBI Protein Information:protein kinase C zeta type
UniProt Protein Name:Protein kinase C zeta type
UniProt Synonym Protein Names:nPKC-zeta
Protein Family:Protein kinase
UniProt Gene Name:PRKCZ  
UniProt Entry Name:KPCZ_HUMAN
Anti-PRKCZ Antibody (CAB5714)Western blot analysis of extracts of various cell lines, using PRKCZ antibody (CAB5714) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-PRKCZ Antibody (CAB5714)Western blot analysis of extracts of various cell lines, using PRKCZ antibody (CAB5714) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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