Cell Biology Antibodies 3

Anti-PRKAR2B Antibody (CAB12751)

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SKU:
CAB12751
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PRKAR2B Antibody
Antibody SKU:CAB12751
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PRKAR2B (NP_002727.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:Jurkat, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PRKAR2B (NP_002727.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSIE IPAG LTEL LQGF TVEV LRHQ PADL LEFA LQHF TRLQ QENE RKGT ARFG HEGR TWGD LGAA AGGG TPSK GVNF AEEP MQSD SEDG EEEE AAPA DAGA
Gene ID:5577
Uniprot:P31323
Cellular Location:Cell membrane, Cytoplasm
Calculated MW:46kDa
Observed MW:50kDa
Synonyms:PRKAR2B, PRKAR2, RII-BETA
Background:cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.
UniProt Protein Function:PKAR2B: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
UniProt Protein Details:

Protein type:Mitochondrial; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 7q22.3

Cellular Component: centrosome; cytoplasm; cytosol; dendritic shaft; dendritic spine; plasma membrane

Molecular Function:cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; protein binding; ubiquitin protein ligase binding

Biological Process: activation of protein kinase A; blood coagulation; G2/M transition of mitotic cell cycle; renal water homeostasis

NCBI Summary:cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol. [provided by RefSeq, Jul 2008]
UniProt Code:P31323
NCBI GenInfo Identifier:206729918
NCBI Gene ID:5577
NCBI Accession:P31323.3
UniProt Secondary Accession:P31323,A4D0R9,
UniProt Related Accession:P31323
Molecular Weight:46kDa
NCBI Full Name:cAMP-dependent protein kinase type II-beta regulatory subunit
NCBI Synonym Full Names:protein kinase cAMP-dependent type II regulatory subunit beta
NCBI Official Symbol:PRKAR2B  
NCBI Official Synonym Symbols:PRKAR2; RII-BETA  
NCBI Protein Information:cAMP-dependent protein kinase type II-beta regulatory subunit
UniProt Protein Name:cAMP-dependent protein kinase type II-beta regulatory subunit
Protein Family:cAMP-dependent protein kinase
UniProt Gene Name:PRKAR2B  
Anti-PRKAR2B Antibody (CAB12751)Western blot analysis of extracts of various cell lines, using PRKAR2B antibody (CAB12751) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.
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