Antibody Name:	Anti-PRKAR2A Antibody [KO Validated]
Antibody SKU:	CAB1531
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PRKAR2A (NP_004148.1).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, SW480, LO2, Mouse lung, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PRKAR2A (NP_004148.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSHI QIPP GLTE LLQG YTVE VLRQ QPPD LVEF AVEY FTRL REAR APAS VLPA ATPR QSLG HPPP EPGP DRVA DAKG DSES EEDE DLEV PVPS RFNR RVSV CAET YNPD EEEE DTDP RVIH PKTD EQRC RLQE ACKD ILLF KNLD QEQL SQVL DAMF ERIV KADE HVID QGDD GDNF YVIE RGTY DILV TKDN QTRS VGQY DNRG SFGE LALM YNTP RAAT IVAT SEGS LWGL DRVT FRRI IVKN NAKK RKMF ESFI ESVP LLKS LEVS ERMK IVDV IGEK IYKD GERI ITQG EKAD SFYI IESG EVSI LIRS RTKS NKDG GNQE VEIA RCHK GQYF GELA LVTN KPRA ASAY AVGD VKCL VMDV QAFE RLLG PCMD IMKR NISH YEEQ LVKM FGSS VDLG NLGQ
Gene ID:	5576
Uniprot:	P13861
Cellular Location:	Cell membrane, Cytoplasm
Calculated MW:	43kDa/45kDa
Observed MW:	46kDa

Synonyms:

PRKAR2A, PKR2, PRKAR2

Background:

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).

UniProt Protein Function:	PKAR2A: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
UniProt Protein Details:	Protein type:Protein kinase, regulatory subunit Chromosomal Location of Human Ortholog: 3p21.3-p21.2 Cellular Component: centrosome; focal adhesion; membrane; cytoplasm; plasma membrane; cytosol; AMP-activated protein kinase complex; cAMP-dependent protein kinase complex Molecular Function:protein binding; cAMP-dependent protein kinase inhibitor activity; ubiquitin protein ligase binding; cAMP-dependent protein kinase regulator activity; cAMP binding Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; phospholipase C activation; water transport; activation of protein kinase A; energy reserve metabolic process; innate immune response; renal water homeostasis; blood coagulation; signal transduction; regulation of insulin secretion; transmembrane transport
NCBI Summary:	cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]
UniProt Code:	P13861
NCBI GenInfo Identifier:	125198
NCBI Gene ID:	5576
NCBI Accession:	P13861.2
UniProt Secondary Accession:	P13861,Q16823,
UniProt Related Accession:	P13861
Molecular Weight:	45,518 Da
NCBI Full Name:	cAMP-dependent protein kinase type II-alpha regulatory subunit
NCBI Synonym Full Names:	protein kinase, cAMP-dependent, regulatory, type II, alpha
NCBI Official Symbol:	PRKAR2A
NCBI Official Synonym Symbols:	PKR2; PRKAR2
NCBI Protein Information:	cAMP-dependent protein kinase type II-alpha regulatory subunit; protein kinase A, RII-alpha subunit; cAMP-dependent protein kinase regulatory subunit RII alpha
UniProt Protein Name:	cAMP-dependent protein kinase type II-alpha regulatory subunit
Protein Family:	CD99 antigen
UniProt Gene Name:	PRKAR2A
UniProt Entry Name:	KAP2_HUMAN

	Western blot analysis of extracts of various cell lines, using PRKAR2A antibody (CAB1531) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Western blot analysis of extracts from normal (control) and PRKAR2A knockout (KO) HeLa cells, using PRKAR2A antibody (CAB1531) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of L929 cells using [KO Validated] PRKAR2A Rabbit pAb (CAB1531) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.