Cell Biology Antibodies 1

Anti-PRKAR1B Antibody (CAB10125)

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SKU:
CAB10125
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PRKAR1B Antibody
Antibody SKU:CAB10125
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 190-290 of human PRKAR1B (NP_002726.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:4000
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, HeLa, Jurkat, HepG2, BT-474, Mouse brain, Mouse lung, Rat brain, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 190-290 of human PRKAR1B (NP_002726.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:WVTN ISEG GSFG ELAL IYGT PRAA TVKA KTDL KLWG IDRD SYRR ILMG STLR KRKM YEEF LSKV SILE SLEK WERL TVAD ALEP VQFE DGEK IVVQ GEPG D
Gene ID:5575
Uniprot:P31321
Cellular Location:Cell membrane
Calculated MW:43kDa
Observed MW:43kDa
Synonyms:PRKAR1B, PRKAR1
Background:The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene.
UniProt Protein Function:PKAR1B: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase. Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Belongs to the cAMP-dependent kinase regulatory chain family.
UniProt Protein Details:

Protein type:Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cytosol

Molecular Function:cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity

Biological Process: activation of protein kinase A; blood coagulation; renal water homeostasis

NCBI Summary:The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2015]
UniProt Code:P31321
NCBI GenInfo Identifier:229463042
NCBI Gene ID:5575
NCBI Accession:P31321.4
UniProt Secondary Accession:P31321,Q8N422,
UniProt Related Accession:P31321
Molecular Weight:43,073 Da
NCBI Full Name:cAMP-dependent protein kinase type I-beta regulatory subunit
NCBI Synonym Full Names:protein kinase cAMP-dependent type I regulatory subunit beta
NCBI Official Symbol:PRKAR1B  
NCBI Official Synonym Symbols:PRKAR1  
NCBI Protein Information:cAMP-dependent protein kinase type I-beta regulatory subunit
UniProt Protein Name:cAMP-dependent protein kinase type I-beta regulatory subunit
Protein Family:cAMP-dependent protein kinase
UniProt Gene Name:PRKAR1B  
UniProt Entry Name:KAP1_HUMAN
Anti-PRKAR1B Antibody (CAB10125)Western blot analysis of extracts of various cell lines, using PRKAR1B antibody (CAB10125) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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