Antibody Name:	Anti-PRKAG1 Antibody
Antibody SKU:	CAB7300
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human PRKAG1 (NP_002724.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, 22Rv1, Mouse kidney, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human PRKAG1 (NP_002724.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	METV ISSD SSPA VENE HPQE TPES NNSV YTSF MKSH RCYD LIPT SSKL VVFD TSLQ VKKA FFAL VTNG VRAA PLWD SKKQ SFVG MLTI TDFI NILH RYYK SALV QIYE LEEH KIET WREV YLQD SFKP LVCI SPNA SLFD AVSS LIRN KIHR LPVI DPES GNTL YILT HKRI LKFL KLFI TEFP KPEF MSKS LEEL QIGT YANI AMVR TTTP VYVA LGIF VQHR VSAL PVVD EKGR VVDI YSKF DVIN LAAE KTYN NLDV SVTK ALQH RSHY FEGV LKCY LHET LETI INRL VEAE VHRL VVVD ENDV VKGI VSLS DILQ ALVL TGGE KKP
Gene ID:	5571
Uniprot:	P54619
Cellular Location:
Calculated MW:	34kDa/37kDa/38kDa
Observed MW:	36kDa/38kDa

Synonyms:

PRKAG1, AMPKG

Background:

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

UniProt Protein Function:	AMPKG1: a non-catalytic subunit of AMPK, a highly conserved kinase of the CAMKL family. The holoenzyme consists of a catalytic subunit (alpha) and two regulatory subunits (beta, gamma). Each subunit exists as multiple genetic isoforms giving rise to 12 different possible combinations of holoenzyme. Plays a key role in regulation of energy homeostasis. Activated by an elevated AMP-ATP ratio due to environmental stress, such as heat shock, hypoxia and ischemia. AMPK phosphorylates and regulates many metabolic enzymes.
UniProt Protein Details:	Protein type:Autophagy; Protein kinase, regulatory subunit Chromosomal Location of Human Ortholog: 12q12-q14 Cellular Component: AMP-activated protein kinase complex; cytosol; membrane; nucleoplasm Molecular Function:ADP binding; AMP binding; AMP-activated protein kinase activity; ATP binding; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase regulator activity; protein binding; protein kinase activity; protein kinase binding Biological Process: cell cycle arrest; macroautophagy; positive regulation of protein kinase activity; protein amino acid phosphorylation; regulation of glycolysis; signal transduction; spermatogenesis
NCBI Summary:	The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:	P54619
NCBI GenInfo Identifier:	1703037
NCBI Gene ID:	5571
NCBI Accession:	P54619.1
UniProt Secondary Accession:	P54619,Q8N7V9, B4DDT7,
UniProt Related Accession:	P54619
Molecular Weight:	38,533 Da
NCBI Full Name:	5'-AMP-activated protein kinase subunit gamma-1
NCBI Synonym Full Names:	protein kinase AMP-activated non-catalytic subunit gamma 1
NCBI Official Symbol:	PRKAG1
NCBI Official Synonym Symbols:	AMPKG
NCBI Protein Information:	5'-AMP-activated protein kinase subunit gamma-1
UniProt Protein Name:	5'-AMP-activated protein kinase subunit gamma-1
Protein Family:	5'-AMP-activated protein kinase
UniProt Gene Name:	PRKAG1
UniProt Entry Name:	AAKG1_HUMAN

	Western blot analysis of extracts of various cell lines, using PRKAG1 antibody (CAB7300) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human stomach using PRKAG1 antibody (CAB7300) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using PRKAG1 antibody (CAB7300) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using PRKAG1 antibody (CAB7300) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using PRKAG1 antibody (CAB7300). Blue: DAPI for nuclear staining.