Cell Biology Antibodies 7
Anti-PRG3 Antibody (CAB16481)
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- SKU:
- CAB16481
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PRG3 Antibody |
| Antibody SKU: | CAB16481 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-225 of human PRG3 (NP_006084.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse spleen, Mouse thymus, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-225 of human PRG3 (NP_006084.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LHLE NDAP HLES LETQ ADLG QDLD SSKE QERD LALT EEVI QAEG EEVK ASAC QDNF EDEE AMES DPAA LDKD FQCP REED IVEV QGSP RCKI CRYL LVRT PKTF AEAQ NVCS RCYG GNLV SIHD FNFN YRIQ CCTS TVNQ AQVW IGGN LRGW FLWK RFCW TDGS HWNF AYWS PGQP GNGQ GSCV ALCT KGGY WRRA QCDK QLPF VCSF |
| Gene ID: | 10394 |
| Uniprot: | Q9Y2Y8 |
| Cellular Location: | Cytoplasmic granule |
| Calculated MW: | 25kDa |
| Observed MW: | 25kDa |
| Synonyms: | PRG3, MBP2, MBPH |
| Background: |
| UniProt Protein Function: | PRG3: Possesses similar cytotoxic and cytostimulatory activities to PRG2/MBP. In vitro, stimulates neutrophil superoxide production and IL8 release, and histamine and leukotriene C4 release from basophils. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 11q12.1 Cellular Component: extracellular region Molecular Function:carbohydrate binding Biological Process: basophil activation; histamine biosynthetic process; leukotriene biosynthetic process; negative regulation of translation; neutrophil activation; neutrophil degranulation; positive regulation of interleukin-8 biosynthetic process; superoxide anion generation |
| UniProt Code: | Q9Y2Y8 |
| NCBI GenInfo Identifier: | 223633965 |
| NCBI Gene ID: | 10394 |
| NCBI Accession: | NP_006084.2 |
| UniProt Related Accession: | Q9Y2Y8 |
| Molecular Weight: | 25kDa |
| NCBI Full Name: | proteoglycan 3 |
| NCBI Synonym Full Names: | proteoglycan 3, pro eosinophil major basic protein 2 |
| NCBI Official Symbol: | PRG3 |
| NCBI Official Synonym Symbols: | MBP2; MBPH |
| NCBI Protein Information: | proteoglycan 3 |
| UniProt Protein Name: | Proteoglycan 3 |
| UniProt Synonym Protein Names: | Eosinophil major basic protein homolog; Prepro-major basic protein homolog; Prepro-MBPH |
| Protein Family: | Proteoglycan |
| UniProt Gene Name: | PRG3 |
 | Western blot analysis of extracts of various cell lines, using PRG3 antibody (CAB16481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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