Cell Biology Antibodies 4
Anti-PREPL Antibody (CAB14348)
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- SKU:
- CAB14348
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PREPL Antibody |
| Antibody SKU: | CAB14348 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 578-727 of human PREPL (NP_006027.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, Mouse heart, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 578-727 of human PREPL (NP_006027.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AVTL EAPF LDVL NTMM DTTL PLTL EELE EWGN PSSD EKHK NYIK RYCP YQNI KPQH YPSI HITA YEND ERVP LKGI VSYT EKLK EAIA EHAK DTGE GYQT PNII LDIQ PGGN HVIE DSHK KITA QIKF LYEE LGLD STSV FEDL KKYL KF |
| Gene ID: | 9581 |
| Uniprot: | Q4J6C6 |
| Cellular Location: | Cytoplasm, cytosol |
| Calculated MW: | 73kDa/76kDa/83kDa |
| Observed MW: | 72kDa |
| Synonyms: | PREPL, CMS22 |
| Background: | The protein encoded by this gene belongs to the prolyl oligopeptidase subfamily of serine peptidases. Mutations in this gene have been associated with hypotonia-cystinuria syndrome, also known as the 2p21 deletion syndrome. Several alternatively spliced transcript variants encoding either the same or different isoforms have been described for this gene. |
| UniProt Code: | Q4J6C6 |
| NCBI GenInfo Identifier: | 121944206 |
| NCBI Gene ID: | 9581 |
| NCBI Accession: | Q4J6C6.1 |
| UniProt Secondary Accession: | Q4J6C6,O43163, Q4J6C3, Q4J6C4, Q4ZG39, Q6ZMW7, Q96DW7 A7E2X6, D6W5A3, |
| UniProt Related Accession: | Q4J6C6 |
| Molecular Weight: | 727 |
| NCBI Full Name: | Prolyl endopeptidase-like |
| NCBI Synonym Full Names: | prolyl endopeptidase-like |
| NCBI Official Symbol: | PREPL |
| NCBI Protein Information: | prolyl endopeptidase-like; putative prolyl oligopeptidase |
| UniProt Protein Name: | Prolyl endopeptidase-like |
| UniProt Synonym Protein Names: | Prolylendopeptidase-like |
| Protein Family: | Prolyl endopeptidase |
| UniProt Gene Name: | PREPL |
| UniProt Entry Name: | PPCEL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PREPL antibody (CAB14348) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of C6 cells using PREPL Polyclonal Antibody (CAB14348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using PREPL Polyclonal Antibody (CAB14348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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