Cell Biology Antibodies 12
Anti-Prefoldin subunit 3 Antibody (CAB8990)
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- SKU:
- CAB8990
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Prefoldin subunit 3 Antibody |
| Antibody SKU: | CAB8990 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human VBP1 (NP_003363.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-251MG, HepG2, 293T, Mouse stomach |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human VBP1 (NP_003363.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAV KDSC GKGE MATG NGRR LHLG IPEA VFVE DVDS FMKQ PGNE TADT VLKK LDEQ YQKY KFME LNLA QKKR RLKG QIPE IKQT LEIL KYMQ KKKE STNS METR FLLA DNLY CKAS VPPT DKVC LWLG ANVM LEYD IDEA QALL EKNL STAT KNLD SLEE DLDF LRDQ FTTT EVNM ARVY NWDV KRRN KDDS TKNK A |
| Gene ID: | 7411 |
| Uniprot: | P61758 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 22kDa |
| Observed MW: | 23kDa |
| Synonyms: | VBP1, HIBBJ46, PFD3, PFDN3, VBP-1 |
| Background: | The protein encoded by this gene interacts with the Von Hippel-Lindau protein to form an intracellular complex. The encoded protein functions as a chaperone protein, and may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm. Alternative splicing and the use of alternate transcription start sites results in multiple transcript variants encoding different protein isoforms. |
| UniProt Protein Function: | Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. |
| NCBI Summary: | The protein encoded by this gene interacts with the Von Hippel-Lindau protein to form an intracellular complex. The encoded protein functions as a chaperone protein, and may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm. Alternative splicing and the use of alternate transcription start sites results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Jan 2015] |
| UniProt Code: | P61758 |
| NCBI GenInfo Identifier: | 746816135 |
| NCBI Gene ID: | 7411 |
| NCBI Accession: | NP_001290472.1 |
| UniProt Secondary Accession: | P61758,O55228, Q15765, Q5JT81, Q86X96, B2R8L5, |
| UniProt Related Accession: | P61758 |
| Molecular Weight: | |
| NCBI Full Name: | prefoldin subunit 3 isoform 2 |
| NCBI Synonym Full Names: | VHL binding protein 1 |
| NCBI Official Symbol: | VBP1 |
| NCBI Official Synonym Symbols: | PFD3; PFDN3; VBP-1; HIBBJ46 |
| NCBI Protein Information: | prefoldin subunit 3 |
| UniProt Protein Name: | Prefoldin subunit 3 |
| UniProt Synonym Protein Names: | HIBBJ46; von Hippel-Lindau-binding protein 1; VBP-1; VHL-binding protein 1 |
| UniProt Gene Name: | VBP1 |
 | Western blot analysis of extracts of various cell lines, using VBP1 antibody (CAB8990) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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