Name: Anti-PRDM5 Antibody (CAB7361)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB7361
Availability: OutOfStock

Antibody Name:	Anti-PRDM5 Antibody
Antibody SKU:	CAB7361
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PRDM5 (NP_061169.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Mouse heart, Mouse lung, Mouse ovary, Rat kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PRDM5 (NP_061169.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLGM YVPD RFSL KSSR VQDG MGLY TARR VRKG EKFG PFAG EKRM PEDL DENM DYRL MWEV RGSK GEVL YILD ATNP RHSN WLRF VHEA PSQE QKNL AAIQ
Gene ID:	11107
Uniprot:	Q9NQX1
Cellular Location:	Nucleus
Calculated MW:	12kDa/58kDa/69kDa/73kDa
Observed MW:	110kDa

Synonyms:	PRDM5, BCS2, PFM2
Background:	The protein encoded by this gene is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis.

UniProt Protein Function:	PRDM5: Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes. May regulate the expression of proteins involved in extracellular matrix development and maintenance, including fibrillar collagens, such as COL4A1 and COL11A1, connective tissue components, such as HAPLN1, and molecules regulating cell migration and adhesion, including EDIL3 and TGFB2. May caused G2/M arrest and apoptosis in cancer cells. Defects in PRDM5 are the cause of Brittle cornea syndrome type 2 (BCS2). A disorder characterized by extreme corneal thinning resulting in corneal rupture after minor trauma, blue sclerae, keratoconus or keratoglobus, hyperelasticity of the skin, and hypermobile joints. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Methyltransferase, protein lysine, predicted; DNA-binding; Transcription factor; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 4q25-q26 Cellular Component: nucleus Molecular Function:protein binding; sequence-specific DNA binding Biological Process: histone deacetylation; histone H3-K9 methylation; mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent Disease: Brittle Cornea Syndrome 2
NCBI Summary:	The protein encoded by this gene is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NQX1
NCBI GenInfo Identifier:	212276458
NCBI Gene ID:	11107
NCBI Accession:	Q9NQX1.2
UniProt Secondary Accession:	Q9NQX1,Q0VAI9, Q0VAJ0, Q6NXQ7,
UniProt Related Accession:	Q9NQX1
Molecular Weight:	58,124 Da
NCBI Full Name:	PR domain zinc finger protein 5
NCBI Synonym Full Names:	PR/SET domain 5
NCBI Official Symbol:	PRDM5
NCBI Official Synonym Symbols:	BCS2; PFM2
NCBI Protein Information:	PR domain zinc finger protein 5
UniProt Protein Name:	PR domain zinc finger protein 5
UniProt Synonym Protein Names:	PR domain-containing protein 5
Protein Family:	PR domain zinc finger protein
UniProt Gene Name:	PRDM5
UniProt Entry Name:	PRDM5_HUMAN

	Western blot analysis of extracts of various cell lines, using PRDM5 antibody (CAB7361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat spleen using PRDM5 antibody (CAB7361) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using PRDM5 antibody (CAB7361) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using PRDM5 antibody (CAB7361) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using PRDM5 antibody (CAB7361). Blue: DAPI for nuclear staining.