Name: Anti-PRDM2 Antibody (CAB13157)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB13157
Availability: OutOfStock

Antibody Name:	Anti-PRDM2 Antibody
Antibody SKU:	CAB13157
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PRDM2 (NP_036363.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, LO2, MCF7, Mouse brain, Mouse liver, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PRDM2 (NP_036363.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ILKG KKFG PFVG DKKK RSQV KNNV YMWE VYYP NLGW MCID ATDP EKGN WLRY VNWA CSGE EQNL FPLE INRA IYYK TLKP IAPG EELL VWYN GEDN PEIA A
Gene ID:	7799
Uniprot:	Q13029
Cellular Location:	Nucleus
Calculated MW:	25kDa/162kDa/166kDa/184kDa/188kDa
Observed MW:	300kDa

Synonyms:

PRDM2, HUMHOXY1, KMT8, KMT8A, MTB-ZF, RIZ, RIZ1, RIZ2

Background:

This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	KSR: a serine/threonine kinase of the Raf family with no demonstrated kinase activity. A location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. Interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. In unstimulated cells, the phosphorylated form is bound to a 14-3-3 protein and sequestered in the cytoplasm. Translocates from the cytoplasm to the cell periphery following TNF treatment. When Bad is available, ceramide signaling through the Ras/Raf cascade can lead to apoptosis. Three alternatively-spliced isoforms have been described.
UniProt Protein Details:	Protein type:Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; RAF family Chromosomal Location of Human Ortholog: 17q11.2 Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum Molecular Function:protein serine/threonine kinase activity; MAP-kinase scaffold activity; protein binding; mitogen-activated protein kinase kinase binding; metal ion binding; ATP binding; protein kinase activity Biological Process: positive regulation of MAPKKK cascade; Ras protein signal transduction; protein amino acid phosphorylation
NCBI Summary:	This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
UniProt Code:	Q13029
NCBI GenInfo Identifier:	20336258
NCBI Gene ID:	7799
NCBI Accession:	NP_036363
UniProt Related Accession:	Q13029
Molecular Weight:
NCBI Full Name:	PR domain zinc finger protein 2 isoform a
NCBI Synonym Full Names:	PR/SET domain 2
NCBI Official Symbol:	PRDM2
NCBI Official Synonym Symbols:	RIZ; KMT8; RIZ1; RIZ2; KMT8A; MTB-ZF; HUMHOXY1
NCBI Protein Information:	PR domain zinc finger protein 2
UniProt Protein Name:	Kinase suppressor of Ras 1
Protein Family:	Kinase suppressor of Ras
UniProt Gene Name:	KSR1
UniProt Entry Name:	KSR1_HUMAN

Western blot analysis of extracts of various cell lines, using PRDM2 antibody (CAB13157) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-PRDM2 Antibody (CAB8565)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)