Immunology Antibodies 2

Anti-PRDM1 Antibody (CAB1960)

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SKU:
CAB1960
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-PRDM1 Antibody
Antibody SKU:CAB1960
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PRDM1 (NP_878911.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:LO2, BxPC-3, K-562, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PRDM1 (NP_878911.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEKI YSRG ELHH FIDG FNEE KSNW MRYV NPAH SPRE QNLA ACQN GMNI YFYT IKPI PANQ ELLV WYCR DFAE RLHY PYPG ELTM MNLT QTQS SLKQ PSTE KNEL CPKN VPKR EYSV KEIL KLDS NPSK GKDL YRSN ISPL TSEK DLDD FRRR GSPE MPFY PRVV YPIR APLP EDFL KASL AYGI ERPT YITR SPIP SSTT PSPS ARSS PDQS LKSS SPHS SPGN TVSP VGPG SQEH RDSY
Gene ID:639
Uniprot:O75626
Cellular Location:Cytoplasm, Nucleus
Calculated MW:76kDa/87kDa/91kDa
Observed MW:100kDa
Synonyms:PRDM1, BLIMP1, PRDI-BF1
Background:This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported.
UniProt Protein Function:PRDM1: a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene and can inhibit virus-mediated IFN-¿ production. Recruits chromatin-modifying enzymes including histone deacetylases and methyltransferases. Drives the maturation of B-lymphocytes into Ig secreting cells. Interacts with PRMT5. Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1. In the B-cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation and in a subset of T-cells suggesting that it may play a wider role in homeostasis and differentiation. Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc, CIITA, Pax5, Spi-B, and Id3.Three isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription regulation; Transcription, coactivator/corepressor; C2H2-type zinc finger protein; Methyltransferase, protein lysine, predicted; DNA-binding

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; nucleoplasm

Molecular Function:protein binding; transcription factor activity

Biological Process: negative regulation of transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:O75626
NCBI GenInfo Identifier:296452869
NCBI Gene ID:639
NCBI Accession:O75626.2
UniProt Secondary Accession:O75626,Q86WM7, B2REA6, E1P5E0,
UniProt Related Accession:O75626
Molecular Weight:76,835 Da
NCBI Full Name:PR domain zinc finger protein 1
NCBI Synonym Full Names:PR/SET domain 1
NCBI Official Symbol:PRDM1  
NCBI Official Synonym Symbols:BLIMP1; PRDI-BF1  
NCBI Protein Information:PR domain zinc finger protein 1
UniProt Protein Name:PR domain zinc finger protein 1
UniProt Synonym Protein Names:BLIMP-1; Beta-interferon gene positive regulatory domain I-binding factor; PR domain-containing protein 1; Positive regulatory domain I-binding factor 1; PRDI-BF1; PRDI-binding factor 1
Protein Family:PR domain zinc finger protein
UniProt Gene Name:PRDM1  
UniProt Entry Name:PRDM1_HUMAN
Anti-PRDM1 Antibody (CAB1960)Western blot analysis of extracts of various cell lines, using PRDM1 antibody (CAB1960) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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