Antibody Name:	Anti-PRC1 Antibody
Antibody SKU:	CAB7029
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 351-620 of human PRC1 (NP_003972.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Jurkat, 293T, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 351-620 of human PRC1 (NP_003972.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LFEG VQKW EETW RLFL EFER KASD PNRF TNRG GNLL KEEK QRAK LQKM LPKL EEEL KARI ELWE QEHS KAFM VNGQ KFME YVAE QWEM HRLE KERA KQER QLKN KKQT ETEM LYGS APRT PSKR RGLA PNTP GKAR KLNT TTMS NATA NSSI RPIF GGTV YHSP VSRL PPSG SKPV AAST CSGK KTPR TGRH GANK ENLE LNGS ILSG GYPG SAPL QRNF SINS VAST YSEF AKDP SLSD SSTV GLQR ELSK ASKS DATS GILN STNI QS
Gene ID:	9055
Uniprot:	O43663
Cellular Location:	Cytoplasm, Midbody, Nucleus, cytoskeleton, spindle pole
Calculated MW:	61kDa/66kDa/70kDa/71kDa
Observed MW:	72kDa

Synonyms:

PRC1, ASE1

Background:

This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	PRC1: translocates to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. Acts as a microtubule-binding and bundling protein both in vivo and vitro. Interacts with the C-terminal Rho-GAP domain and the basic region of MgcRacGAP. The interaction with MgcRacGAP inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. Interacts separately via its N-terminal region with the C-terminus of CENPE, KIF4A and KIF23 during late mitosis.
UniProt Protein Details:	Protein type:Cell cycle regulation; Cytoskeletal; Microtubule-binding; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 15q26.1 Cellular Component: nucleoplasm; microtubule cytoskeleton; spindle pole; spindle microtubule; cytoplasm; plasma membrane; spindle; nucleus Molecular Function:identical protein binding; protein binding; kinesin binding; microtubule binding; protein kinase binding Biological Process: mitotic spindle elongation; cytokinesis
NCBI Summary:	This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
UniProt Code:	O43663
NCBI GenInfo Identifier:	215273870
NCBI Gene ID:	9055
NCBI Accession:	O43663.2
UniProt Related Accession:	O43663
Molecular Weight:	61kDa; 66kDa; 70kDa; 71kDa
NCBI Full Name:	Protein regulator of cytokinesis 1
NCBI Synonym Full Names:	protein regulator of cytokinesis 1
NCBI Official Symbol:	PRC1
NCBI Official Synonym Symbols:	ASE1
NCBI Protein Information:	protein regulator of cytokinesis 1
UniProt Protein Name:	Protein regulator of cytokinesis 1
Protein Family:	Protein regulator of cytokinesis
UniProt Gene Name:	PRC1
UniProt Entry Name:	PRC1_HUMAN

	Western blot analysis of extracts of various cell lines, using PRC1 antibody (CAB7029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat spleen using PRC1 Rabbit pAb (CAB7029) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human thyroid cancer using PRC1 Rabbit pAb (CAB7029) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using PRC1 Rabbit pAb (CAB7029) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using PRC1 Rabbit pAb (CAB7029) at dilution of 1:100 (40x lens).