Cell Biology Antibodies 12

Anti-PPP4R1 Antibody (CAB8361)

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SKU:
CAB8361
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IP
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PPP4R1 Antibody
Antibody SKU:CAB8361
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 324-647 of human PPP4R1 (NP_001035847.1).
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:293T, U-251MG, BT-474, K-562, SKOV3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 324-647 of human PPP4R1 (NP_001035847.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NPSS SGQY FKEE SKSS EEMS VENK NRTR DQEA PEDV QVRP EDTP SDLS VSNS SVIL ENTM EDHA AEAS GKPL GEIS VPLD SSLL CTLS SESH QEAA SNEN DKKP GNYK SMLR PEVG TTSQ DSAL LDQE LYNS FHFW RTPL PEID LDIE LEQN SGGK PSPE GPEE ESEG PVPS SPNI TMAT RKEL EEMI ENLE PHID DPDV KAQV EVLS AALR ASSL DAHE ETIS IEKR SDLQ DELD INEL PNCK INQE DSVP LISD AVEN MDST LHYI HSDS DLSN NSSF SPDE ERRT KVQD VVPQ ALLD QYLS MTDP SRAQ TVDT EIAK HCAY
Gene ID:9989
Uniprot:Q8TF05
Cellular Location:
Calculated MW:105kDa/107kDa
Observed MW:130kDa
Synonyms:PPP4R1, MEG1, PP4(Rmeg), PP4R1
Background:This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants.
NCBI Summary:This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
UniProt Code:Q8TF05
NCBI GenInfo Identifier:108936953
NCBI Gene ID:9989
NCBI Accession:NP_001035847
UniProt Related Accession:Q8TF05
Molecular Weight:
NCBI Full Name:serine/threonine-protein phosphatase 4 regulatory subunit 1 isoform a
NCBI Synonym Full Names:protein phosphatase 4 regulatory subunit 1
NCBI Official Symbol:PPP4R1  
NCBI Official Synonym Symbols:MEG1; PP4R1; PP4(Rmeg)  
NCBI Protein Information:serine/threonine-protein phosphatase 4 regulatory subunit 1
UniProt Protein Name:Serine/threonine-protein phosphatase 4 regulatory subunit 1
UniProt Gene Name:PPP4R1  
UniProt Entry Name:PP4R1_HUMAN
Anti-PPP4R1 Polyclonal Antibody (CAB8361)Western blot analysis of extracts of various cell lines, using PPP4R1 antibody (CAB8361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-PPP4R1 Polyclonal Antibody (CAB8361)Immunohistochemistry of paraffin-embedded human liver using PPP4R1 antibody (CAB8361) at dilution of 1:100 (40x lens).
Anti-PPP4R1 Polyclonal Antibody (CAB8361)Immunohistochemistry of paraffin-embedded human stomach using PPP4R1 antibody (CAB8361) at dilution of 1:100 (40x lens).
Anti-PPP4R1 Polyclonal Antibody (CAB8361)Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug PPP4R1 antibody (CAB8361). Western blot was performed from the immunoprecipitate using PPP4R1 antibody (CAB8361) at a dilition of 1:1000.
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