Antibody Name:	Anti-PPP2R3B Antibody
Antibody SKU:	CAB8489
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 356-575 of human PPP2R3B (NP_037371.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HeLa, SW620, BT-474, SKOV3, mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 356-575 of human PPP2R3B (NP_037371.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FSGA VTRG RKVQ KEGK ISYA DFVW FLIS EEDK KTPT SIEY WFRC MDLD GDGA LSMF ELEY FYEE QCRR LDSM AIEA LPFQ DCLC QMLD LVKP RTEG KITL QDLK RCKL ANVF FDTF FNIE KYLD HEQK EQIS LLRD GDSG GPEL SDWE KYAA EEYD ILVA EETA GEPW EDGF EAEL SPVE QKLS ALRS PLAQ RPFF EAPS PLGA VDLY EYAC GDED LEPL
Gene ID:	28227
Uniprot:	Q9Y5P8
Cellular Location:	Nucleus
Calculated MW:	20kDa/65kDa
Observed MW:	80kDa

Synonyms:

PPP2R3B, NYREN8, PPP2R3L, PPP2R3LY, PR48, PR70

Background:

Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the beta subfamily of regulatory subunit B''.

UniProt Protein Function:	PPP2R3B: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit Chromosomal Location of Human Ortholog: Xp22.33; Yp11.3 Cellular Component: nucleoplasm; nucleus; protein phosphatase type 2A complex Molecular Function:phosphoprotein phosphatase activity; protein binding; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity Biological Process: cell cycle arrest; protein amino acid dephosphorylation
NCBI Summary:	Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the beta subfamily of regulatory subunit B''. [provided by RefSeq, Apr 2010]
UniProt Code:	Q9Y5P8
NCBI GenInfo Identifier:	158523346
NCBI Gene ID:	28227
NCBI Accession:	Q9Y5P8.2
UniProt Secondary Accession:	Q9Y5P8,Q6P4G9, Q7RTT1, Q96H01,
UniProt Related Accession:	Q9Y5P8
Molecular Weight:	20,111 Da
NCBI Full Name:	Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta
NCBI Synonym Full Names:	protein phosphatase 2 regulatory subunit B''beta
NCBI Official Symbol:	PPP2R3B
NCBI Official Synonym Symbols:	PR48; NYREN8; PPP2R3L; PPP2R3LY
NCBI Protein Information:	serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta
UniProt Protein Name:	Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta
UniProt Synonym Protein Names:	PP2A subunit B isoform PR48; Protein phosphatase 2A 48 kDa regulatory subunit
Protein Family:	Serine/threonine-protein phosphatase
UniProt Gene Name:	PPP2R3B
UniProt Entry Name:	P2R3B_HUMAN

	Western blot analysis of extracts of various cell lines, using PPP2R3B antibody (CAB8489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Western blot analysis of extracts of mouse skeletal muscle, using PPP2R3B antibody (CAB8489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.