Name: Anti-PPP2R2B Antibody (CAB10494)
Brand: Cell Death Antibodies 1
SKU: CAB10494
Availability: OutOfStock

Antibody Name:	Anti-PPP2R2B Antibody
Antibody SKU:	CAB10494
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 160-260 of human PPP2R2B (NP_858060.2).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, SH-SY5Y, HepG2, Mouse brain, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 160-260 of human PPP2R2B (NP_858060.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KIRW LPQQ NAAY FLLS TNDK TVKL WKVS ERDK RPEG YNLK DEEG RLRD PATI TTLR VPVL RPMD LMVE ATPR RVFA NAHT YHIN SISV NSDY ETYM SADD L
Gene ID:	5521
Uniprot:	Q00005
Cellular Location:	Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, cytoskeleton
Calculated MW:	50kDa/51kDa/52kDa/58kDa/62kDa
Observed MW:	52kDa

Synonyms:

PPP2R2B, B55BETA, PP2AB55BETA, PP2ABBETA, PP2APR55B, PP2APR55BETA, PR2AB55BETA, PR2ABBETA, PR2APR55BETA, PR52B, PR55-BETA, PR55BETA, SCA12

Background:

The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in this gene cause autosomal dominant spinocerebellar ataxia 12 (SCA12), a disease caused by degeneration of the cerebellum, sometimes involving the brainstem and spinal cord, and in resulting in poor coordination of speech and body movements. Multiple alternatively spliced variants, which encode different isoforms, have been identified for this gene. The 5' UTR of some of these variants includes a CAG trinucleotide repeat sequence (7-28 copies) that can be expanded to 55-78 copies in cases of SCA12.

UniProt Protein Function:	PPP2R2B: the B regulatory subunit of PP2A. May modulate substrate selectivity and catalytic activity, and directs the localization of the catalytic enzyme to particular subcellular compartments. PPP2R2B promotes autophagy and oxidative stress induced cell death that is related to apoptosis. The PP2A holoenzyme consists of a common heterodimeric core enzyme composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (subunit A), that associates with a variety of regulatory subunits. Three families of regulatory subunits associate with the core dimmer: B (R2/PR55/B55), B¿ (R5/B56), and B¿ (R3/PR72/PR130/PR59). Five isoforms of the human protein are produced by alternative splicing. Isoform 1 (Bbeta1) localizes within the cytoplasm. Isoform 2 (Bbeta2) localizes to both cytosolic and mitochondrial compartments under basal conditions, relocating to the mitochondrial compartment during apoptosis. Its targeting to the outer mitochondrial membrane (OMM) involves an association with import receptors of the TOM complex and is required to promote proapoptotic activity. The N-terminal 26 residues of isoform 2 constitute a cryptic mitochondrial matrix import signal that is necessary and sufficient for targeting the PP2A holoenzyme to the OMM. The last WD repeat of isoform 2 constitutes a mitochondrial stop-transfer domain that prevents PPP2R2B matrix translocation and signal sequence cleavage. Defects in PPP2R2B are the cause of spinocerebellar ataxia type 12 (SCA12).
UniProt Protein Details:	Protein type:Mitochondrial; Apoptosis; Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit Chromosomal Location of Human Ortholog: 5q32 Cellular Component: mitochondrial outer membrane; mitochondrion Molecular Function:protein binding Disease: Spinocerebellar Ataxia 12
NCBI Summary:	The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in this gene cause autosomal dominant spinocerebellar ataxia 12 (SCA12), a disease caused by degeneration of the cerebellum, sometimes involving the brainstem and spinal cord, and in resulting in poor coordination of speech and body movements. Multiple alternatively spliced variants, which encode different isoforms, have been identified for this gene. The 5' UTR of some of these variants includes a CAG trinucleotide repeat sequence (7-28 copies) that can be expanded to 55-78 copies in cases of SCA12. [provided by RefSeq, Jul 2016]
UniProt Code:	Q00005
NCBI GenInfo Identifier:	231446
NCBI Gene ID:	5521
NCBI Accession:	Q00005.1
UniProt Secondary Accession:	Q00005,A6NEJ2, A8K102, B3KPD0, B7Z2F2, B7Z304, D3DQF7 D3DQF8, G3V149,
UniProt Related Accession:	Q00005
Molecular Weight:	62,505 Da
NCBI Full Name:	Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform
NCBI Synonym Full Names:	protein phosphatase 2 regulatory subunit Bbeta
NCBI Official Symbol:	PPP2R2B
NCBI Official Synonym Symbols:	PR52B; SCA12; B55BETA; PR55BETA; PP2ABBETA; PP2APR55B; PR2ABBETA; PR55-BETA; PP2AB55BETA; PR2AB55BETA; PP2APR55BETA; PR2APR55BETA
NCBI Protein Information:	serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform
UniProt Protein Name:	Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform
UniProt Synonym Protein Names:	PP2A subunit B isoform B55-beta; PP2A subunit B isoform PR55-beta; PP2A subunit B isoform R2-beta; PP2A subunit B isoform beta
Protein Family:	Serine/threonine-protein phosphatase
UniProt Gene Name:	PPP2R2B
UniProt Entry Name:	2ABB_HUMAN

Western blot analysis of extracts of various cell lines, using PPP2R2B antibody (CAB10494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

PPP2R2B Antibody (PACO04690)

PPP2R2B Antibody (PACO11433)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)