Antibody Name:	Anti-PPP2CB Antibody
Antibody SKU:	CAB3122
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human PPP2CB (NP_001009552.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, 293T, Mouse brain, Mouse lung, Mouse spleen, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human PPP2CB (NP_001009552.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDDK AFTK ELDQ WVEQ LNEC KQLN ENQV RTLC EKAK EILT KESN VQEV RCPV TVCG DVHG QFHD LMEL FRIG GKSP DTNY LFMG DYVD RGYY SVET VTLL VALK VRYP ERIT ILRG NHES RQIT QVYG FYDE CLRK YGNA NVWK YFTD LFDY LPLT ALVD GQIF CLHG GLSP SIDT LDHI RALD RLQE VPHE GPMC DLLW SDPD DRGG WGIS PRGA GYTF GQDI SETF NHAN GLTL VSRA HQLV MEGY NWCH DRNV VTIF SAPN YCYR CGNQ AAIM ELDD TLKY SFLQ FDPA PRRG EPHV TRRT PDYF L
Gene ID:	5516
Uniprot:	P62714
Cellular Location:	Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, spindle pole
Calculated MW:	35kDa
Observed MW:	36KDa

Synonyms:

PPP2CB, PP2Abeta, PP2CB

Background:

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit.

UniProt Protein Function:	PPP2CB: one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits.
UniProt Protein Details:	Protein type:Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.16 Chromosomal Location of Human Ortholog: 8p12 Cellular Component: spindle pole; protein phosphatase type 2A complex; nucleus; cytosol; chromosome, pericentric region Molecular Function:protein C-terminus binding; protein binding; metal ion binding; protein serine/threonine phosphatase activity Biological Process: response to antibiotic; proteasomal ubiquitin-dependent protein catabolic process; apoptotic mitochondrial changes; fibroblast growth factor receptor signaling pathway; response to hydrogen peroxide; regulation of gene expression; negative regulation of Ras protein signal transduction; protein amino acid dephosphorylation
NCBI Summary:	This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. [provided by RefSeq, Mar 2010]
UniProt Code:	P62714
NCBI GenInfo Identifier:	50402236
NCBI Gene ID:	5516
NCBI Accession:	P62714.1
UniProt Secondary Accession:	P62714,P67775, P62715, P63330, P62716 P63331,
UniProt Related Accession:	P62714
Molecular Weight:	35kDa
NCBI Full Name:	Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
NCBI Synonym Full Names:	protein phosphatase 2 catalytic subunit beta
NCBI Official Symbol:	PPP2CB
NCBI Official Synonym Symbols:	PP2CB; PP2Abeta
NCBI Protein Information:	serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
UniProt Protein Name:	Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
Protein Family:	PP2A regulatory
UniProt Gene Name:	PPP2CB
UniProt Entry Name:	PP2AB_HUMAN

	Western blot analysis of extracts of various cell lines, using PPP2CB antibody (CAB3122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat kidney using PPP2CB antibody (CAB3122) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using PPP2CB antibody (CAB3122) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using PPP2CB antibody (CAB3122) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using PPP2CB antibody (CAB3122) at dilution of 1:100. Blue: DAPI for nuclear staining.