Antibody Name:	Anti-PPP1R8 Antibody
Antibody SKU:	CAB6701
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human PPP1R8 (NP_612568.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, PC-12, HeLa, HepG2, 293T, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human PPP1R8 (NP_612568.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGGE DDEL KGLL GLPE EETE LDNL TEFN TAHN KRIS TLTI EEGN LDIQ RPKR KRKN SRVT FSED DEII NPED VDPS VGRF RNMV QTAV VPVK KKRV EGPG SLGL EESG SRRM QNFA FSGG LYGG LPPT HSEA GSQP HGIH GTAL IGGL PMPY PNLA PDVD LTPV VPSA VNMN PAPN PAVY NPEA VNEP KKKK YAKE AWPG KKPT PSLL I
Gene ID:	5511
Uniprot:	Q12972
Cellular Location:	Cytoplasm, Nucleus, Nucleus speckle
Calculated MW:	13kDa/22kDa/38kDa
Observed MW:	40kDa

Synonyms:

PPP1R8, ARD-1, ARD1, NIPP-1, NIPP1, PRO2047

Background:

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

UniProt Protein Function:	NIPP-1: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Inhibitor; Protein phosphatase, regulatory subunit; Ribonuclease; EC 3.1.4.-; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1p35.3 Cellular Component: nucleoplasm; spliceosome; cytoplasm; nuclear speck; nucleus Molecular Function:protein binding; ribonuclease E activity; DNA binding; protein phosphatase type 1 regulator activity; RNA binding; endonuclease activity; type 1 serine/threonine specific protein phosphatase inhibitor activity Biological Process: cell proliferation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; RNA catabolic process; negative regulation of catalytic activity; mRNA processing
NCBI Summary:	This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]
UniProt Code:	Q12972
NCBI GenInfo Identifier:	19863082
NCBI Gene ID:	5511
NCBI Accession:	Q12972.2
UniProt Secondary Accession:	Q12972,Q5TEJ2, Q5TEJ4, Q5TIF2, Q6PKF6, Q9UBH1, Q9UBZ0
UniProt Related Accession:	Q12972
Molecular Weight:	351
NCBI Full Name:	Nuclear inhibitor of protein phosphatase 1
NCBI Synonym Full Names:	protein phosphatase 1, regulatory subunit 8
NCBI Official Symbol:	PPP1R8
NCBI Official Synonym Symbols:	ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
NCBI Protein Information:	nuclear inhibitor of protein phosphatase 1; RNase E; activator of RNA decay; nuclear subunit of PP-1; protein phosphatase 1 regulatory subunit 8; nuclear inhibitor of protein phosphatase-1 alpha; protein phosphatase 1, regulatory (inhibitor) subunit 8
UniProt Protein Name:	Nuclear inhibitor of protein phosphatase 1
UniProt Synonym Protein Names:	Protein phosphatase 1 regulatory inhibitor subunit 8Including the following 1 domains:Activator of RNA decay (EC:3.1.4.-)Alternative name(s):ARD-1
Protein Family:	Nuclear inhibitor of protein phosphatase
UniProt Gene Name:	PPP1R8
UniProt Entry Name:	PP1R8_HUMAN

	Western blot analysis of extracts of various cell lines, using PPP1R8 antibody (CAB6701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using PPP1R8 Antibody (CAB6701) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using PPP1R8 Antibody (CAB6701) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using PPP1R8 Antibody (CAB6701) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using PPP1R8 antibody (CAB6701).