Metabolism Antibodies 1

Anti-PPP1R3A Antibody (CAB10171)

(No reviews yet) Write a Review
SKU:
CAB10171
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-PPP1R3A Antibody
Antibody SKU:CAB10171
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 900-1080 of human PPP1R3A (NP_002702.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 900-1080 of human PPP1R3A (NP_002702.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SAFN SDTN RAPQ NSSP FSKH HTEI SVST NEQA IAVE NAVT TMAS QPIS TKSE NICN STRE IQGI EKHP YPES KPEE VSRS SGIV TSGS RKER CIGQ IFQT EEYS VEKS LGPM ILIN KPLE NMEE ARHE NEGL VSSG QSLY TSGE KESD SSAS TSLP VEES QAQG NESL FSKY TNSK IPYF L
Gene ID:5506
Uniprot:Q16821
Cellular Location:Membrane, Single-pass membrane protein
Calculated MW:8kDa/125kDa
Observed MW:150kDa
Synonyms:PPP1R3A, GM, PP1G, PPP1R3
Background:The glycogen-associated form of protein phosphatase-1 (PP1) derived from skeletal muscle is a heterodimer composed of a 37-kD catalytic subunit and a 124-kD targeting and regulatory subunit. This gene encodes the regulatory subunit which binds to muscle glycogen with high affinity, thereby enhancing dephosphorylation of glycogen-bound substrates for PP1 such as glycogen synthase and glycogen phosphorylase kinase.
UniProt Protein Function:PPP1R3A: Seems to act as a glycogen-targeting subunit for PP1. PP1 is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Plays an important role in glycogen synthesis but is not essential for insulin activation of glycogen synthase. Interacts with PPP1CC catalytic subunit of PP1, and associates with glycogen. Skeletal muscle and heart. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 7q31.1

Disease: Lipodystrophy, Familial Partial, Type 3

NCBI Summary:The glycogen-associated form of protein phosphatase-1 (PP1) derived from skeletal muscle is a heterodimer composed of a 37-kD catalytic subunit and a 124-kD targeting and regulatory subunit. This gene encodes the regulatory subunit which binds to muscle glycogen with high affinity, thereby enhancing dephosphorylation of glycogen-bound substrates for PP1 such as glycogen synthase and glycogen phosphorylase kinase. [provided by RefSeq, Jul 2008]
UniProt Code:Q16821
NCBI GenInfo Identifier:298286906
NCBI Gene ID:5506
NCBI Accession:Q16821.3
UniProt Secondary Accession:Q16821,O43476, Q75LN8, Q7KYM8, Q86UI6, A0AVQ2, A4D0T6
UniProt Related Accession:Q16821
Molecular Weight:8,187 Da
NCBI Full Name:Protein phosphatase 1 regulatory subunit 3A
NCBI Synonym Full Names:protein phosphatase 1 regulatory subunit 3A
NCBI Official Symbol:PPP1R3A  
NCBI Official Synonym Symbols:GM; PP1G; PPP1R3  
NCBI Protein Information:protein phosphatase 1 regulatory subunit 3A
UniProt Protein Name:Protein phosphatase 1 regulatory subunit 3A
UniProt Synonym Protein Names:Protein phosphatase 1 glycogen-associated regulatory subunit; Protein phosphatase type-1 glycogen targeting subunit; RG1
Protein Family:Protein phosphatase 1 regulatory
UniProt Gene Name:PPP1R3A  
UniProt Entry Name:PPR3A_HUMAN
Anti-PPP1R3A Antibody (CAB10171)Western blot analysis of extracts of mouse liver, using PPP1R3A antibody (CAB10171) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
View AllClose

0 Reviews

View AllClose

Related Products