Cell Biology Antibodies 1

Anti-PPP1R14A Antibody (CAB10160)

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SKU:
CAB10160
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-PPP1R14A Antibody
Antibody SKU:CAB10160
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human PPP1R14A (NP_150281.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, Mouse lung, Mouse brain, Mouse spleen, Rat lung, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human PPP1R14A (NP_150281.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAQ RLGK RVLS KLQS PSRA RGPG GSPG GLQK RHAR VTVK YDRR ELQR RLDV EKWI DGRL EELY RGME ADMP DEIN IDEL LELE SEEE RSRK IQGL LKSC GKPV EDFI QELL AKLQ GLHR QPGL RQPS PSHD GSLS PLQD RART AHP
Gene ID:94274
Uniprot:Q96A00
Cellular Location:Cytoplasm
Calculated MW:13kDa/16kDa
Observed MW:19KDa
Synonyms:PPP1R14A, CPI-17, CPI17, PPP1INL
Background:The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
UniProt Protein Function:PPP1R14A: a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity. Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2 ) concentration. Involved in the regulation of cell morphology, re-organisation of microfilaments, and cell spreading. Also expressed in human platelets and in brain where it may play a role in cerebellar long-term synaptic depression. Phosphorylation by ILK or Rho kinase enhances its ability to inhibit PP-1A.
UniProt Protein Details:

Protein type:Inhibitor; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytosol

Molecular Function:protein phosphatase inhibitor activity

Biological Process: negative regulation of catalytic activity; regulation of phosphorylation; small GTPase mediated signal transduction

NCBI Summary:The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:Q96A00
NCBI GenInfo Identifier:55583974
NCBI Gene ID:94274
NCBI Accession:Q96A00.1
UniProt Secondary Accession:Q96A00,Q7Z4X7, Q96S54,
UniProt Related Accession:Q96A00
Molecular Weight:13,480 Da
NCBI Full Name:Protein phosphatase 1 regulatory subunit 14A
NCBI Synonym Full Names:protein phosphatase 1 regulatory inhibitor subunit 14A
NCBI Official Symbol:PPP1R14A  
NCBI Official Synonym Symbols:CPI17; CPI-17; PPP1INL  
NCBI Protein Information:protein phosphatase 1 regulatory subunit 14A
UniProt Protein Name:Protein phosphatase 1 regulatory subunit 14A
UniProt Synonym Protein Names:17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17
UniProt Gene Name:PPP1R14A  
UniProt Entry Name:PP14A_HUMAN
Anti-PPP1R14A Antibody (CAB10160)Western blot analysis of extracts of various cell lines, using PPP1R14A Rabbit pAb (CAB10160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
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system_update_altDatasheetHuman PPP1R14A Recombinant Protein Protein Phosphatase 1 Regulatory Subunit 14A (PPP1R14A) belongs to the PP1 inhibitor family. PPP1R14A is mapped to chromosome 19q13.13