Antibody Name:	Anti-PPP1CC Antibody [KO Validated]
Antibody SKU:	CAB4035
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP ChIP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human PPP1CC (NP_002701.1).

Application:	WB IHC IP ChIP
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 ChIP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HL-60, Mouse thymus, Mouse spleen, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human PPP1CC (NP_002701.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADL DKLN IDSI IQRL LEVR GSKP GKNV QLQE NEIR GLCL KSRE IFLS QPIL LELE APLK ICGD IHGQ YYDL LRLF EYGG FPPE SNYL FLGD YVDR GKQS LETI CLLL AYKI KYPE NFFL LRGN HECA SINR IYGF YDEC KRRY NIKL WKTF TDCF NCLP IAAI VDEK IFCC HGGL SPDL QSME QIRR IMRP TDVP DQGL LCDL LWSD PDKD VLGW GEND RGVS FTFG AEVV AKFL HKHD LDLI CRAH QVVE DGYE FFAK RQLV TLFS APNY CGEF DNAG AMMS VDET LMCS FQIL KPAE KKKP NATR PVTP PRGM ITKQ AKK
Gene ID:	5501
Uniprot:	P36873
Cellular Location:	Chromosome, Cleavage furrow, Cytoplasm, Midbody, Mitochondrion, Nucleus, Nucleus speckle, centromere, kinetochore, nucleolus, nucleoplasm
Calculated MW:	36kDa/38kDa
Observed MW:	38kDa

Synonyms:

PPP1CC, PP-1G, PP1C, PPP1G

Background:

The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PPP1CC: is a catalytic subunit of protein phosphatase 1 (PP1), a ubiquitous cytosolic serine-threonine phosphatase. Composed a catalytic subunit (PPP1CA, PPP1CB or PPP1CC) which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting (or regulatory) subunits: PPP1R12A and PPP1R12B mediate binding to myosin; PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen; and PPP1R15A and PPP1R15B mediate binding to EIF2S1. PP1 is essential for cell division, plays critical roles in many cellular processes including glycogen metabolism, muscle contractility and protein synthesis, and is involved in the regulation of ionic conductances and long-term synaptic plasticity. Inhibits the synthesis of proteins involved in synaptic plasticity. Binds to the transcription factor cyclic AMP-dependent response element binding (CREB) and dephosphorylates it. May regulate chromatin condensation through regulation of histone H3 phosphorylation. Differentially expressed in gastric cancer. May participate in the neurodegenerative progress in Alzheimer's disease. Down-regulated in lung squamous cell carcinoma.
UniProt Protein Details:	Protein type:EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor); Nucleolus Chromosomal Location of Human Ortholog: 12q24.1-q24.2 Cellular Component: focal adhesion; protein complex; mitochondrion; cytoplasm; nucleolus; nuclear speck; midbody; cytosol; nucleus; cleavage furrow Molecular Function:protein binding; metal ion binding; phosphoric monoester hydrolase activity; protein serine/threonine phosphatase activity; phosphoprotein phosphatase activity; protein kinase binding Biological Process: glycogen metabolic process; cell division; transforming growth factor beta receptor signaling pathway; triacylglycerol catabolic process; mitotic cell cycle; circadian regulation of gene expression; regulation of circadian rhythm; protein amino acid dephosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; entrainment of circadian clock by photoperiod
NCBI Summary:	The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:	P36873
NCBI GenInfo Identifier:	548573
NCBI Gene ID:	5501
NCBI Accession:	P36873.1
UniProt Related Accession:	P36873
Molecular Weight:	323
NCBI Full Name:	Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
NCBI Synonym Full Names:	protein phosphatase 1, catalytic subunit, gamma isozyme
NCBI Official Symbol:	PPP1CC
NCBI Official Synonym Symbols:	PP1C; PP-1G; PPP1G
NCBI Protein Information:	serine/threonine-protein phosphatase PP1-gamma catalytic subunit; serine/threonine phosphatase 1 gamma; protein phosphatase 1C catalytic subunit; protein phosphatase 1, catalytic subunit, gamma isoform
UniProt Protein Name:	Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
UniProt Synonym Protein Names:	Protein phosphatase 1C catalytic subunit
UniProt Gene Name:	PPP1CC
UniProt Entry Name:	PP1G_HUMAN

	Western blot analysis of extracts of various cell lines, using PPP1CC antibody (CAB4035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and PPP1CC knockout (KO) HeLa cells, using PPP1CC antibody (CAB4035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human gastric cancer using PPP1CC antibody (CAB4035) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using PPP1CC antibody (CAB4035) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using PPP1CC antibody (CAB4035) at dilution of 1:100 (40x lens).