Name: Anti-PPM1G Antibody (CAB12173)
Brand: Cell Biology Antibodies 2
SKU: CAB12173
Availability: OutOfStock

Antibody Name:	Anti-PPM1G Antibody
Antibody SKU:	CAB12173
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 297-546 of human PPM1G (NP_817092.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	A-431, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 297-546 of human PPM1G (NP_817092.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DTEE AEED DEEE EEEM MVPG MEGK EEPG SDSG TTAV VALI RGKQ LIVA NAGD SRCV VSEA GKAL DMSY DHKP EDEV ELAR IKNA GGKV TMDG RVNG GLNL SRAI GDHF YKRN KNLP PEEQ MISA LPDI KVLT LTDD HEFM VIAC DGIW NVMS SQEV VDFI QSKI SQRD ENGE LRLL SSIV EELL DQCL APDT SGDG TGCD NMTC IIIC FKPR NTAE LQPE SGKR KLEE VLST EGAE ENGN SDKK KKAK RD
Gene ID:	5496
Uniprot:	O15355
Cellular Location:	Cytoplasm, Lipid-anchor, Membrane
Calculated MW:	59kDa
Observed MW:	75kDa

Synonyms:

PPM1G, PP2CG, PP2CGAMMA, PPP2CG

Background:

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression.

UniProt Protein Function:	PPM1G: is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]
UniProt Protein Details:	Protein type:Spliceosome; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2p23.3 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:protein binding; metal ion binding; protein serine/threonine phosphatase activity Biological Process: protein amino acid dephosphorylation; cell cycle arrest
NCBI Summary:	The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]
UniProt Code:	O15355
NCBI GenInfo Identifier:	3122589
NCBI Gene ID:	5496
NCBI Accession:	O15355.1
UniProt Related Accession:	O15355
Molecular Weight:
NCBI Full Name:	Protein phosphatase 1G
NCBI Synonym Full Names:	protein phosphatase, Mg2+/Mn2+ dependent 1G
NCBI Official Symbol:	PPM1G
NCBI Official Synonym Symbols:	PP2CG; PPP2CG; PP2CGAMMA
NCBI Protein Information:	protein phosphatase 1G
UniProt Protein Name:	Protein phosphatase 1G
UniProt Synonym Protein Names:	Protein phosphatase 1C; Protein phosphatase 2C isoform gamma; PP2C-gamma; Protein phosphatase magnesium-dependent 1 gamma
Protein Family:	Protein phosphatase
UniProt Gene Name:	PPM1G
UniProt Entry Name:	PPM1G_HUMAN

Western blot analysis of extracts of various cell lines, using PPM1G antibody (CAB12173) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

PPM1G Antibody (PACO11410)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)