Cell Cycle Antibodies 1

Anti-PPM1D Antibody (CAB6204)

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SKU:
CAB6204
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-PPM1D Antibody
Antibody SKU:CAB6204
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PPM1D (NP_003611.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, HeLa, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PPM1D (NP_003611.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAGL YSLG VSVF SDQG GRKY MEDV TQIV VEPE PTAE EKPS PRRS LSQP LPPR PSPA ALPG GEVS GKGP AVAA REAR DPLP DAGA SPAP SRCC RRRS SVAF FAVC DGHG GREA AQFA REHL WGFI KKQK GFTS SEPA KVCA AIRK GFLA CHLA MWKK LAEW PKTM TGLP STSG TTAS VVII RGMK MYVA HVGD SGVV LGIQ DDPK DDFV RAVE VTQD HKPE LPKE RERI EGLG GSVM NKSG VNRV VWKR PR
Gene ID:8493
Uniprot:O15297
Cellular Location:
Calculated MW:47kDa/66kDa
Observed MW:67kDa
Synonyms:PPM1D, PP2C-DELTA, WIP1, IDDGIP
Background:The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.
UniProt Protein Function:PPM1D: a magnesium-dependent Ser/Thr phosphatase. A p53-induced phosphatase that interacts with the nuclear isoform of uracil DNA glycosylase, UNG2, and suppresses base excision repair. Might contribute to growth inhibitory pathways activated in response to DNA damage in a p53-dependent manner.
UniProt Protein Details:

Protein type:Cell cycle regulation; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: nucleus

Molecular Function:mitogen-activated protein kinase binding; protein binding; protein serine/threonine phosphatase activity

Biological Process: negative regulation of cell proliferation; protein amino acid dephosphorylation; response to radiation

Disease: Breast Cancer

NCBI Summary:The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008]
UniProt Code:O15297
NCBI GenInfo Identifier:10720176
NCBI Gene ID:8493
NCBI Accession:O15297.1
UniProt Secondary Accession:O15297,Q53XP4, Q6P991, Q8IVR6,
UniProt Related Accession:O15297
Molecular Weight:47,211 Da
NCBI Full Name:Protein phosphatase 1D
NCBI Synonym Full Names:protein phosphatase, Mg2+/Mn2+ dependent 1D
NCBI Official Symbol:PPM1D  
NCBI Official Synonym Symbols:WIP1; PP2C-DELTA  
NCBI Protein Information:protein phosphatase 1D
UniProt Protein Name:Protein phosphatase 1D
UniProt Synonym Protein Names:Protein phosphatase 2C isoform delta; PP2C-delta; Protein phosphatase magnesium-dependent 1 delta; p53-induced protein phosphatase 1
Protein Family:Protein phosphatase
UniProt Gene Name:PPM1D  
UniProt Entry Name:PPM1D_HUMAN
Anti-PPM1D Antibody (CAB6204)Western blot analysis of extracts of various cell lines, using PPM1D antibody (CAB6204) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 60s.
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