Antibody Name:	Anti-PPL Antibody
Antibody SKU:	CAB6950
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PPL (NP_002696.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse lung, Mouse heart, Rat spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PPL (NP_002696.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNSL FRKR NKGK YSPT VQTR SISN KELS ELIE QLQK NADQ VEKN IVDT EAKM QSDL ARLQ EGRQ PEHR DVTL QKVL DSEK LLYV LEAD AAIA KHMK HPQG DMIA EDIR QLKE RVTN LRGK HKQI YRLA VK
Gene ID:	5493
Uniprot:	O60437
Cellular Location:	Cell junction, Cell membrane, Cytoplasm, Mitochondrion, Nucleus, cytoskeleton, desmosome
Calculated MW:	204kDa
Observed MW:	205kDa

Synonyms:

PPL

Background:

The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.

UniProt Protein Function:	periplakin: Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling. Belongs to the plakin or cytolinker family.
UniProt Protein Details:	Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: desmosome; cytoskeleton; mitochondrion; plasma membrane; nucleus Molecular Function:protein binding; structural constituent of cytoskeleton Biological Process: keratinization
NCBI Summary:	The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. [provided by RefSeq, Jul 2008]
UniProt Code:	O60437
NCBI GenInfo Identifier:	317373534
NCBI Gene ID:	5493
NCBI Accession:	O60437.4
UniProt Related Accession:	O60437
Molecular Weight:	~ 205kDa
NCBI Full Name:	Periplakin
NCBI Synonym Full Names:	periplakin
NCBI Official Symbol:	PPL
NCBI Protein Information:	periplakin
UniProt Protein Name:	Periplakin
UniProt Synonym Protein Names:	190 kDa paraneoplastic pemphigus antigen; 195 kDa cornified envelope precursor protein
Protein Family:	Periplakin
UniProt Gene Name:	PPL
UniProt Entry Name:	PEPL_HUMAN

	Western blot analysis of extracts of various cell lines, using PPL antibody (CAB6950) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunofluorescence analysis of MCF7 cells using PPL antibody (CAB6950). Blue: DAPI for nuclear staining.