Cell Biology Antibodies 11

Anti-PPIB Antibody (CAB7713)

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SKU:
CAB7713
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PPIB Antibody
Antibody SKU:CAB7713
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 34-216 of human PPIB (NP_000933.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, 22Rv1, HepG2, THP-1, Mouse liver, Mouse kidney, Mouse brain, Mouse lung, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 34-216 of human PPIB (NP_000933.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DEKK KGPK VTVK VYFD LRIG DEDV GRVI FGLF GKTV PKTV DNFV ALAT GEKG FGYK NSKF HRVI KDFM IQGG DFTR GDGT GGKS IYGE RFPD ENFK LKHY GPGW VSMA NAGK DTNG SQFF ITTV KTAW LDGK HVVF GKVL EGME VVRK VEST KTDS RDKP LKDV IIAD CGKI EVEK PFAI AKE
Gene ID:5479
Uniprot:P23284
Cellular Location:Endoplasmic reticulum lumen, Melanosome
Calculated MW:23kDa
Observed MW:24kDa
Synonyms:PPIB, CYP-S1, CYPB, HEL-S-39, OI9, SCYLP
Background:The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta.
UniProt Protein Function:PPIB: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Defects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9). OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients. Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
UniProt Protein Details:

Protein type:Isomerase; Chaperone; EC 5.2.1.8; Secreted; Cyclophilin; Secreted, signal peptide; RNA-binding

Chromosomal Location of Human Ortholog: 15q21-q22

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; focal adhesion; melanosome; membrane; nucleus; perinuclear region of cytoplasm; smooth endoplasmic reticulum

Molecular Function:collagen binding; peptide binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; protein complex binding; unfolded protein binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of multicellular organism growth; protein peptidyl-prolyl isomerization; protein stabilization

Disease: Osteogenesis Imperfecta, Type Ix

NCBI Summary:The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009]
UniProt Code:P23284
NCBI GenInfo Identifier:215273869
NCBI Gene ID:5479
NCBI Accession:P23284.2
UniProt Secondary Accession:P23284,Q6IBH5, Q9BVK5, A8K534,
UniProt Related Accession:P23284
Molecular Weight:23,743 Da
NCBI Full Name:Peptidyl-prolyl cis-trans isomerase B
NCBI Synonym Full Names:peptidylprolyl isomerase B
NCBI Official Symbol:PPIB  
NCBI Official Synonym Symbols:OI9; CYPB; SCYLP; CYP-S1; HEL-S-39  
NCBI Protein Information:peptidyl-prolyl cis-trans isomerase B
UniProt Protein Name:Peptidyl-prolyl cis-trans isomerase B
UniProt Synonym Protein Names:CYP-S1; Cyclophilin B; Rotamase B; S-cyclophilin; SCYLP
Protein Family:Probable peptidyl-prolyl cis-trans isomerase
UniProt Gene Name:PPIB  
UniProt Entry Name:PPIB_HUMAN
Anti-PPIB Antibody (CAB7713)Western blot analysis of extracts of various cell lines, using PPIB antibody (CAB7713) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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