Name: Anti-PPFIBP1 Antibody (CAB16456)
Brand: Cell Biology Antibodies 7
SKU: CAB16456
Availability: OutOfStock

Antibody Name:	Anti-PPFIBP1 Antibody
Antibody SKU:	CAB16456
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 846-1005 of human PPFIBP1 (NP_003613.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-375, HeLa, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 846-1005 of human PPFIBP1 (NP_003613.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LLNI PPNK TLLR RHLA THFN LLIG AEAQ HQKR DAME LPDY VLLT ATAK VKPK KLAF SNFG NLRK KKQE DGEE YVCP MELG QASG SASK KGFK PGLD MRLY EEDD LDRL EQME DSEG TVRQ IGAF SEGI NNLT HMLK EDDM FKDF AARS PSAS ITDE DSNV
Gene ID:	8496
Uniprot:	Q86W92
Cellular Location:
Calculated MW:	18kDa/96kDa/110kDa/113kDa/114kDa
Observed MW:	114kDa

Synonyms:

PPFIBP1, L2, SGT2, hSGT2, hSgt2p

Background:

The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein was found to interact with S100A4, a calcium-binding protein related to tumor invasiveness and metastasis. In vitro experiment demonstrated that the interaction inhibited the phosphorylation of this protein by protein kinase C and protein kinase CK2. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

UniProt Protein Function:	liprin beta 1: a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein was found to interact with S100A4, a calcium-binding protein related to tumor invasiveness and metastasis. In vitro experiment demonstrated that the interaction inhibited the phosphorylation of this protein by protein kinase C and protein kinase CK2. Five alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 12p12.1 Cellular Component: focal adhesion; plasma membrane Biological Process: cell adhesion
NCBI Summary:	The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein was found to interact with S100A4, a calcium-binding protein related to tumor invasiveness and metastasis. In vitro experiment demonstrated that the interaction inhibited the phosphorylation of this protein by protein kinase C and protein kinase CK2. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	Q86W92
NCBI GenInfo Identifier:	90185247
NCBI Gene ID:	8496
NCBI Accession:	Q86W92.2
UniProt Secondary Accession:	Q86W92,O75336, Q86X70, Q9NY03, Q9ULJ0,
UniProt Related Accession:	Q86W92
Molecular Weight:	1011
NCBI Full Name:	Liprin-beta-1
NCBI Synonym Full Names:	PTPRF interacting protein, binding protein 1 (liprin beta 1)
NCBI Official Symbol:	PPFIBP1
NCBI Official Synonym Symbols:	L2; SGT2; hSGT2; hSgt2p
NCBI Protein Information:	liprin-beta-1; liprin-beta 1; liprin related protein; PTPRF-interacting protein-binding protein 1; protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1; protein-tyrosine phosphatase receptor-type f polypeptide-interacting protein-binding protein 1
UniProt Protein Name:	Liprin-beta-1
UniProt Synonym Protein Names:	Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1; PTPRF-interacting protein-binding protein 1; hSGT2
Protein Family:	Liprin
UniProt Gene Name:	PPFIBP1
UniProt Entry Name:	LIPB1_HUMAN

Western blot analysis of extracts of various cell lines, using PPFIBP1 antibody (CAB16456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

PPFIBP1 Antibody (PACO01022)

PPFIBP1 Antibody (PACO11394)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)