Name: Anti-PPFIA1 Antibody (CAB10388)
Brand: Cell Biology Antibodies 1
SKU: CAB10388
Availability: OutOfStock

Antibody Name:	Anti-PPFIA1 Antibody
Antibody SKU:	CAB10388
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 660-800 of human PPFIA1 (NP_003617.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	U-87MG, 293T, MCF7, DU145

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 660-800 of human PPFIA1 (NP_003617.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IESR VGSG SLDN LGRF RSMS SIPP YPAS SLAS SSPP GSGR STPR RIPH SPAR EVDR LGVM TLLP PSRE EVRD DKTT IKCE TSPP SSPR ALRL DRLH KGAL HTVS HEDI RDIR NSTG SQDG PVSN PSSS NSSQ DSLH KAPK K
Gene ID:	8500
Uniprot:	Q13136
Cellular Location:	Cytoplasm
Calculated MW:	133kDa/135kDa
Observed MW:	136kDa

Synonyms:

PPFIA1, LIP.1, LIP1, LIPRIN

Background:

The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. This protein binds to the intracellular membrane-distal phosphatase domain of tyrosine phosphatase LAR, and appears to localize LAR to cell focal adhesions. This interaction may regulate the disassembly of focal adhesion and thus help orchestrate cell-matrix interactions. Alternatively spliced transcript variants encoding distinct isoforms have been described.

UniProt Protein Function:	liprin alpha 1: a ubiqutous member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. This protein binds to the intracellular membrane-distal phosphatase domain of tyrosine phosphatase LAR, and appears to localize LAR to cell focal adhesions. This interaction may regulate the disassembly of focal adhesion and thus help orchestrate cell-matrix interactions. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cell adhesion Chromosomal Location of Human Ortholog: 11q13.3 Cellular Component: focal adhesion; cytoplasm; presynaptic active zone; cytosol Molecular Function:signal transducer activity; protein binding Biological Process: synaptic transmission; negative regulation of stress fiber formation; glutamate secretion; cell-matrix adhesion; neurotransmitter secretion; signal transduction
NCBI Summary:	The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. This protein binds to the intracellular membrane-distal phosphatase domain of tyrosine phosphatase LAR, and appears to localize LAR to cell focal adhesions. This interaction may regulate the disassembly of focal adhesion and thus help orchestrate cell-matrix interactions. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13136
NCBI GenInfo Identifier:	42558969
NCBI Gene ID:	8500
NCBI Accession:	Q13136.1
UniProt Related Accession:	Q13136
Molecular Weight:
NCBI Full Name:	Liprin-alpha-1
NCBI Synonym Full Names:	PTPRF interacting protein alpha 1
NCBI Official Symbol:	PPFIA1
NCBI Official Synonym Symbols:	LIP1; LIP.1; LIPRIN
NCBI Protein Information:	liprin-alpha-1
UniProt Protein Name:	Liprin-alpha-1
UniProt Synonym Protein Names:	LAR-interacting protein 1; LIP-1; Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-1; PTPRF-interacting protein alpha-1
Protein Family:	Lipoyl synthase
UniProt Gene Name:	PPFIA1
UniProt Entry Name:	LIPA1_HUMAN

Western blot analysis of extracts of various cell lines, using PPFIA1 antibody (CAB10388) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

PPFIA1 Antibody (PACO11392)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)