Metabolism Antibodies 2
Anti-PPAT Antibody (CAB6698)
- SKU:
- CAB6698
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-PPAT Antibody |
| Antibody SKU: | CAB6698 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPAT (NP_002694.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, COS-7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPAT (NP_002694.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MELE ELGI REEC GVFG CIAS GEWP TQLD VPHV ITLG LVGL QHRG QESA GIVT SDGS SVPT FKSH KGMG LVNH VFTE DNLK KLYV SNLG IGHT RYAT TGKC ELEN CQPF VVET LHGK IAVA HNGE LVNA ARLR KKLL RHGI GLST SSDS EMIT QLLA YTPP QEQD DTPD WVAR IKNL MKEA PTAY SLLI MHRD VIYA VRDP YGNR PLCI GRLI PVSD INDK EKKT SETE GWVV SSES CSFL SIGA RYYR EVLP GEIV EISR HNVQ TLDI ISRS EGNP VAFC |
| Gene ID: | 5471 |
| Uniprot: | Q06203 |
| Cellular Location: | |
| Calculated MW: | 57kDa |
| Observed MW: | 55kDa |
| Synonyms: | PPAT, ATASE, GPAT, PRAT |
| Background: | The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. |
| UniProt Protein Function: | ATASE: Homotetramer. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - purine; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; EC 2.4.2.14 Chromosomal Location of Human Ortholog: 4q12 Cellular Component: cytosol Molecular Function:amidophosphoribosyltransferase activity Biological Process: purine nucleotide biosynthetic process; purine ribonucleoside monophosphate biosynthetic process |
| NCBI Summary: | The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011] |
| UniProt Code: | Q06203 |
| NCBI GenInfo Identifier: | 548638 |
| NCBI Gene ID: | 5471 |
| NCBI Accession: | Q06203.1 |
| UniProt Related Accession: | Q06203 |
| Molecular Weight: | 57,399 Da |
| NCBI Full Name: | Amidophosphoribosyltransferase |
| NCBI Synonym Full Names: | phosphoribosyl pyrophosphate amidotransferase |
| NCBI Official Symbol: | PPAT |
| NCBI Official Synonym Symbols: | GPAT; PRAT; ATASE |
| NCBI Protein Information: | amidophosphoribosyltransferase |
| UniProt Protein Name: | Amidophosphoribosyltransferase |
| UniProt Synonym Protein Names: | Glutamine phosphoribosylpyrophosphate amidotransferase; GPAT |
| Protein Family: | Pyridoxamine--pyruvate transaminase |
| UniProt Gene Name: | PPAT |
| UniProt Entry Name: | PUR1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PPAT antibody (CAB6698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using PPAT Polyclonal Antibody (CAB6698) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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