Name: Anti-PPA2 Antibody (CAB15819)
Brand: Cell Biology Antibodies 6
SKU: CAB15819
Availability: OutOfStock

Antibody Name:	Anti-PPA2 Antibody
Antibody SKU:	CAB15819
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 33-100 of human PPA2 (NP_008834.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, A-549, OVCAR3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 33-100 of human PPA2 (NP_008834.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ALYH TEER GQPC SQNY RLFF KNVT GHYI SPFH DIPL KVNS KEEN GIPM KKAR NDEY ENLF NMIV EIPR
Gene ID:	27068
Uniprot:	Q9H2U2
Cellular Location:	Mitochondrion
Calculated MW:	18kDa/25kDa/34kDa/37kDa/39kDa
Observed MW:	38kDa

Synonyms:

PPA2, HSPC124, SCFAI, SCFI, SID6-306

Background:

The protein encoded by this gene is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

UniProt Protein Function:	PPA2: is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Mitochondrial; Hydrolase; EC 3.6.1.1; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 4q25 Cellular Component: mitochondrial matrix Molecular Function:inorganic diphosphatase activity; magnesium ion binding; protein serine/threonine phosphatase activity Biological Process: tRNA aminoacylation for protein translation; dephosphorylation; gene expression
NCBI Summary:	The protein encoded by this gene is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9H2U2
NCBI GenInfo Identifier:	77812678
NCBI Gene ID:	27068
NCBI Accession:	NP_008834.3
UniProt Secondary Accession:	Q9H2U2,Q4W5E9, Q6PG51, Q8TBW0, Q96E55, Q9H0T0, Q9NX37 Q9P033, Q9ULX0, B4DLP7, F8WDN9, I6L9B6,
UniProt Related Accession:	Q9H2U2
Molecular Weight:	25,992 Da
NCBI Full Name:	inorganic pyrophosphatase 2, mitochondrial isoform 2
NCBI Synonym Full Names:	pyrophosphatase (inorganic) 2
NCBI Official Symbol:	PPA2
NCBI Official Synonym Symbols:	HSPC124; SID6-306
NCBI Protein Information:	inorganic pyrophosphatase 2, mitochondrial
UniProt Protein Name:	Inorganic pyrophosphatase 2, mitochondrial
UniProt Synonym Protein Names:	Pyrophosphatase SID6-306; Pyrophosphate phospho-hydrolase 2; PPase 2
Protein Family:	Soluble inorganic pyrophosphatase
UniProt Gene Name:	PPA2
UniProt Entry Name:	IPYR2_HUMAN

Western blot analysis of extracts of various cell lines, using PPA2 antibody (CAB15819) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

PPA2 Antibody (PACO11382)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)