Cell Biology Antibodies 4
Anti-PPA1 Antibody (CAB14050)
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- SKU:
- CAB14050
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PPA1 Antibody |
| Antibody SKU: | CAB14050 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PPA1 (NP_066952.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, SKOV3, U-251MG, SW620, 293T, HepG2, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PPA1 (NP_066952.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSGF STEE RAAP FSLE YRVF LKNE KGQY ISPF HDIP IYAD KDVF HMVV EVPR WSNA KMEI ATKD PLNP IKQD VKKG KLRY VANL FPYK GYIW NYGA IPQT WEDP GHND KHTG CCGD NDPI DVCE IGSK VCAR GEII GVKV LGIL AMID EGET DWKV IAIN VDDP DAAN YNDI NDVK RLKP GYLE ATVD WFRR YKVP DGKP ENEF AFNA EFKD KDFA IDII KSTH DHWK ALVT KKTN GKGI SCMN TTLS ESPF KCDP DAAR AIVD ALPP PCES ACTV PTDV DKWF HHQK N |
| Gene ID: | 5464 |
| Uniprot: | Q15181 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 32kDa |
| Observed MW: | 37kDa |
| Synonyms: | PPA1, HEL-S-66p, IOPPP, PP, PP1, SID6-8061 |
| Background: | The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. |
| UniProt Protein Function: | PPA1: is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Hydrolase; EC 3.6.1.1; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 10q11.1-q24 Cellular Component: cytoplasm; cytosol Molecular Function:inorganic diphosphatase activity; magnesium ion binding Biological Process: tRNA aminoacylation for protein translation; gene expression; phosphate metabolic process |
| NCBI Summary: | The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15181 |
| NCBI GenInfo Identifier: | 8247940 |
| NCBI Gene ID: | 5464 |
| NCBI Accession: | Q15181.2 |
| UniProt Secondary Accession: | Q15181,Q2M348, Q5SQT7, Q6P7P4, Q9UQJ5, Q9Y5B1, |
| UniProt Related Accession: | Q15181 |
| Molecular Weight: | 289 |
| NCBI Full Name: | Inorganic pyrophosphatase |
| NCBI Synonym Full Names: | pyrophosphatase (inorganic) 1 |
| NCBI Official Symbol: | PPA1 |
| NCBI Official Synonym Symbols: | PP; PP1; IOPPP; HEL-S-66p; SID6-8061 |
| NCBI Protein Information: | inorganic pyrophosphatase; PPase; pyrophosphatase 1; inorganic diphosphatase; inorganic pyrophosphatase 1; diphosphate phosphohydrolase; pyrophosphate phospho-hydrolase; cytosolic inorganic pyrophosphatase; epididymis secretory sperm binding protein Li 66p |
| UniProt Protein Name: | Inorganic pyrophosphatase |
| UniProt Synonym Protein Names: | Pyrophosphate phospho-hydrolase; PPase |
| Protein Family: | Soluble inorganic pyrophosphatase |
| UniProt Gene Name: | PPA1 |
| UniProt Entry Name: | IPYR_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PPA1 antibody (CAB14050) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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