Epigenetics & Nuclear Signaling Antibodies 2

Anti-POU5F1B Antibody (CAB15705)

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SKU:
CAB15705
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-POU5F1B Antibody
Antibody SKU:CAB15705
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human POU5F1B (NP_001153014.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:LO2, 293T, K-562
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human POU5F1B (NP_001153014.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAGH LASD FAFS PPPG GGGD GPWG AEPG WVDP LTWL SFQG PPGG PGIG PGVG PGSE VWGI PPCP PPYE LCGG MAYC GPQV GVGL VPQG GLET SQPE SEAG VGVE SNSN GASP EPCT VPPG AVKL EKEK LEQN PEKS QDIK
Gene ID:5462
Uniprot:Q06416
Cellular Location:Nucleus
Calculated MW:38kDa
Observed MW:38kDa
Synonyms:POU5F1B, OCT4-PG1, OCT4PG1, OTF3C, OTF3P1, POU5F1P1, POU5F1P4, POU5FLC20, POU5FLC8
Background:This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1, however, it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor, has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development.
UniProt Protein Function:POU5F1B: Shows weak transcriptional activator activity. Belongs to the POU transcription factor family. Class- 5 subfamily.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 8q24.21

Molecular Function:transcription factor activity

NCBI Summary:This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1, however, it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor, has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development. [provided by RefSeq, Apr 2009]
UniProt Code:Q06416
NCBI GenInfo Identifier:357528826
NCBI Gene ID:5462
NCBI Accession:Q06416.2
UniProt Secondary Accession:Q06416,D5K9S4, D5K9S9, D5K9T0, D5K9T1, D5K9T3, D5K9U3 D5K9U6, D5K9V4, D5K9V6, D5K9W0, D5K9W2,
UniProt Related Accession:Q06416
Molecular Weight:38,588 Da
NCBI Full Name:Putative POU domain, class 5, transcription factor 1B
NCBI Synonym Full Names:POU class 5 homeobox 1B
NCBI Official Symbol:POU5F1B  
NCBI Official Synonym Symbols:OTF3C; OTF3P1; OCT4PG1; OCT4-PG1; POU5F1P1; POU5F1P4; POU5FLC8; POU5FLC20  
NCBI Protein Information:putative POU domain, class 5, transcription factor 1B
UniProt Protein Name:Putative POU domain, class 5, transcription factor 1B
UniProt Synonym Protein Names:Oct4-pg1; Octamer-binding protein 3-like; Octamer-binding transcription factor 3-like
Protein Family:Putative POU domain, class 5, transcription factor
UniProt Gene Name:POU5F1B  
UniProt Entry Name:P5F1B_HUMAN
Anti-POU5F1B Antibody (CAB15705)Western blot analysis of extracts of various cell lines, using POU5F1B antibody (CAB15705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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